How Does Enzyme Concentrations Affect The Rate Of A Chemical Reaction?

Enzymes are biological catalysts, which means it decreases activation energy in reactions. The lower activation energy in a reaction, the faster the reaction rate. Many enzymes alter their shape when they bind to the activation site. This is called induced fit, meaning for the enzyme to work to its full potential it has to change shape to binding substrate. The location of enzyme’s activation site is on the surface of the enzyme, where the binding of substrates take place. Enzyme activity can be influenced by a variety of environmental factors. If the concentration of enzyme is low, and there is a great deal of substrate, then increasing enzyme concentration results in more molecules available to convert substrates to products. Thus, increasing enzyme concentration can increase reaction rate. If substrate concentrations are low, and many of the existing enzymes are idle because of a lack of substrate, then adding enzyme will have no effect on reaction rate. Enzyme concentration affects the enzyme activity, because the more enzyme concentration the faster the reaction rate, until it hits it’s limiting factor. When substrate concentration is increased, it also increases rate of reaction. Temperature plays an important

Used to see if the temperature of the water is at 37oc – 40oc and if

Enzyme can be defined as a protein molecule that is a biological catalyst. (Ophardt, 2003) Catalyst increases the speed of a reaction but does not have to use anything to help increase the speed. An enzyme can be determined by their properties. Enzyme are a substrate specific, substrate connects to an enzyme at the location of an active site. Enzyme is not used in a reaction and enzyme function in a good condition at the optimum temperatures and pH. (Ahmez2005) Peroxidase is a type of enzyme which is used in the experiment.

Question: How does changing enzyme concentration or temperature affect the reaction time of enzyme activity?

Enzymes are defined as catalysts that speed up chemical reactions but remain the same themselves. The shape of an enzyme enables it to receive one type of molecule and that specific molecule will fit into the enzyme’s shape. Where a substance fits into an enzyme is called the active site and the substance that fits into the active site is called a substrate. Several factors affect enzymes and the rate of their reactions. Temperature, pH, enzyme concentration, substrate concentration, and the presence of any inhibitors or activators can all affect enzymes. Temperature can affect enzymes because if the temperature gets too high, it can cause the enzyme to denature. pH can affect an enzyme by changing the shape of the enzyme or the charge properties of the substrate so that either the substrate cannot bind to the active site or it cannot undergo catalysis. Every enzyme has an ideal pH that it will strive in. Increasing substrate concentration increases the rate of reaction because more substrate molecules will be interacting and colliding with enzyme molecules, so more product will be formed. Inhibitors can affect enzymes and the rate of their reactions by either slowing down or stopping catalysis. The three types of inhibitors include competitive, non-competitive, and substrate inhibition.

PURPOSE: Measure the effects of changes in catalase concentration, substrate concentration, and salinity on the reaction rates of an enzyme.

The motive of this lab is to attain a better understanding of enzyme activity by timing chemical reactions in certain temperatures and pH levels. Enzymes act as catalysts that help speed up reactions. Without these enzymes chemical reactions in metabolism would be backed up. There are two factors that affect an enzyme’s reaction rate: temperature and pH levels. In this label we will be testing different pH levels and temperatures to see which ones cause the most reactions.

Organisms cannot rely entirely on spontaneous reactions to produce all the materials necessary for life. These reactions occur much too slowly. To produce these materials quicker, cells rely on enzymes, biological catalysts, to speed up these reactions without being consumed. (General Biology I, Martineau, Dean, Gilliland, & Soderstrom, Lab Manual, 2017, 43). To produce these materials quicker, the activation reaction much be lowered, a very important part of this lab. Each enzyme acts on a specific molecule, or set of molecules, called a substrate (43). The enzyme binds to this substrate, forming an enzyme-substrate complex. An enzyme is a protein whose structure is determined by the sequence of amino acids groups that

An Enzyme is a protein, which is capable of starting a chemical reaction, which involves the formation or breakage of chemical bonds. A substrate is the surface or material on or from which an organism lives, grows, or obtains its nourishment. In this case it is hydrogen peroxide. This lab report will be explaining the experiment held to understand the effects of the changes in the amount of substrate on the enzyme’s reaction.

To understand how and why the experiment was performed, one must understand what enzymes and substrates are. Enzymes are defined as proteins that are capable of speeding up a chemical reaction by reducing the amount of activation energy needed to catalyze that reaction (Raven, Johnson and Mason 2014). Enzymes regulate these biochemical processes

In this lab we learned about what influences enzyme activity. We learned many terms and concepts in this lab. Enzymes decreases the amount of energy needed in a reaction. Catalyst speeds up reaction. A substrate is what the material with which catalyst reacts. A product is the modification of the substrate. This was a very informative and good lab.

Living organisms must undergo chemical reactions of cellular metabolism to maintain life. This process is performed with the help of the active site found on the enzyme. The active site is sensible to changes that are made to the enzyme, so when a factor that affects the enzyme is present, the reaction rate will be affected. In this experiment the effects of enzyme concentrations, substrate concentrations, temperature concentrations and adding an inhibitor on the enzyme’s reaction rate will be tested. In activity one, if the enzyme concentration is fairly low, then adding more enzyme concentration will cause the substrates to attach onto the enzyme and be changed into the products. In activity two, if the substrate concentration is not restricted,

Science Coursework – What factors effect the rate of reaction between magnesium and hydrochloric acid? Background Knowledge There are four factors that can affect the rate of reaction and they all rely on the collision theory. This is basically how hard and how often particles collide with each other. The more and harder they collide, the faster the reaction time will be.

The concentration of PNP formed increase with time, The reason behind this is because enzymes are powerful catalysts. They can accelerate the reactions by a factor of as much as a million or more. In this experiment, with the lapse of time, more substrate binds to the enzyme’s active site of which the surface is lined with amino acid residue with substitute groups that bind the substrate and catalyse its chemical transformation. Enzyme reduced the activation energy of this reaction. This enzyme-catalysed reaction takes a different 'route'. The enzyme and substrate form a reaction intermediate. Its formation has a lower activation energy than the reaction between reactants without a catalyst. Therefore more PNP formed.

Enzymes are proteins that act as catalysts and help reactions take place. In short, enzymes reduce the energy needed for a reaction to take place, permitting a reaction to take place more easily. Some enzymes are shape specific and reduce the energy for certain reactions. Enzymes have unique folds of the amino acid chain which result in specifically shaped active sites (Frankova Fry 2013). When substrates fit in the active site of an enzyme, then it is able to catalyze the reaction. Enzyme activity is affected by the concentrations of the enzymes and substrate present (Worthington 2010). As the incidence of enzyme increases, the rate of reaction increases. Additionally, as the incidence of substrate increases so does the rate of reaction.