How Does Temperature Affect Bacteria's Growth?

Essentially the growth and metabolism of any micro-organisms are profoundly affected by the temperature. For example, for Saccharomyces it’s said to increase the production of alcohol increases with temperature up to 40° (Brown 1914). Any organisms (in order to survive) require adenosine triphosphate (ATP), ATP is produced using cellular respiration. Cellular respiration are metabolic reactions that take place in order to produce ATP, which is essential for any cellular activities whether it is movement, work or temperature maintenance. Cellular respiration involves glycolysis followed by citric acid cycle including the Krebs cycle and oxidative phosphorylation. The most amount of ATP is produced in the Krebs cycle

The conditions needed for the growth of micro-organisms are: Micro - organisms need food to survive. They like high protein food to survive, eg. Poultry & fish. Most micro - organisms need warmth & grow best at 20-40c. They need moisture to multiply. They need air to multiply, though some can without. A single Micro-organism becomes two every twenty minutes.

All bacteria need time to be able to multiply and it has been observed that generally bacteria divide into two every twenty minutes.

this means that is the optimal temperature, but in bacterial occur in less time than in fungal

Abstract: The objective of this experiment is to find out what the optimal growth environment is for V. natriegens out of three different environments. The first is a Brain Heart Infusion broth (BHI) that contains 50 mM of NaCl. The second is BHI containing 250 mM of NaCl. The third is BHI containing 1000 mM NaCl. In order to obtain this, a growth curve graph must be constructed for each different environment, and the generation time of the bacteria in each environment must be calculated. The optimal environment for V. natriegens was found to be the BHI with 250 mM of NaCl.

When reflecting back to experiment 3, Aseptic Technique and Culturing Microbes, I realized the large amount of microorganisms that can be found in everyday life. Many different types are found with in the human body. Theses experiments focused on two types of bacteria. First was Staphylococcus epidermidis, found on the skin, and second was Lactobacillus acidophilus, found in the gastrointestinal tract. Both have similar needs for growth when it comes to temperature, however, different growth environments are used.

A few different factors that affect bacterial growth are the availability of resources and nutrients, temperature and pH. (Act For Libraries) stated in the above paragraph, once the resources and nutrients are

For the experiment, the changes of temperature on anaerobic fermentation the process in which cells undergo respiration without oxygen in Saccharomyces cerevisiae was observed. The purpose of this experiment was to test the effect of four different temperatures on the rate of carbon dioxide production in yeast by measuring the fermentation rate. Saccharomyces cereviviae, also known as Baker 's yeast, is a unicellular, eukaryotic sac fungus and is good for this experiment because of its characteristic of alcohol fermentation. It was hypothesized that fermentation increases with increased temperature to a point of 37°C; above that point, enzyme denaturing will occur and fermentation will decrease. The group was able to document the carbon dioxide production and mark each of the temperature intervals which were tested at temperatures 4°C (refrigerator temperature), 23°C (Room temperature), 37°C (Human body temperature) and 65° Celsius (Equal to 150°F). The experiment was conducted by pouring yeast solution with 2% glucose in fermentation tubes, placing the tubes in the appropriate incubation temperature, marking the rise of the gas bubbles in the fermentation tubes which indicated carbon dioxide production. The results of this experiment were not supported by the hypothesis, creating different results from what was predicted. It is important to understand the fermentation rate of yeast so

Grow in very diverse conditions, which explain way they are found nearly everywhere on Earth. Although bacteria are good at adapting to their environments certain conditions promote bacterial growth, more than others . These conditions include temperature , moisture, and environmental oxygen. Understanding the conditions for bacterial growth can potentially help reduce the risk for bacterial growth can potentially help reduce the risk for bacterial infections and food poisoning. Most disease causing bacteria thrive in warm temperature, especially those close to body temperature. The human body , therefore , provides an ideal enviroment for many types of bacteria to grow. Bacteria need water (moisture) to grow and die without a water source,

During the lag phase there is no increase in cell numbers, although the bacteria are synthesizing enzymes present in their environment in preparation for the exponential phase. During the exponential or logarithmic phase, the bacterial population grows at a rate that doubles the population during the generation time. The stationary phase incurs neither an increase nor a decrease in the cell population. The population growth cannot continue at the exponential rate since the nutrient supplies have been depleted and waste products have accumulated. The final phase of the bacterial population growth curve is the death phase, during which more cells die than are replaced by new cells.

Being able to control bacterial growth is something that is important in our everyday lives. As shown in the previous labs, bacteria can grow and create colonies extremely quickly especially in the right environments. By acknowledging this, it is then important to get an understanding of how bacterial growth can be controlled by humans. To control microorganisms it means to inhibit their growth (static) and or kill them (cidal) (Kenneth Todar, 2015); therefore since focusing on bacteria the terms bactericidal and bacteriostatic are both extremely important for this lab. One broad method we will use to control bacterial growth is heat. The amount of heat needed to control bacterial growth is different for different species of bacteria (Kenneth Todar, 2015). Bacteria can also respond differently depending if moist heating method such as an autoclave with steam is used, or a dry heating method such as inoculating a loop over a fire is used (Kenneth Todar, 2015). UV works by damaging the cells DNA, without proper DNA, the cells will die and the object

They curry and cinnamon oil ended up producing more bacteria than the controlled, which had neither curry nor cinnamon oil. My hypothesis of, if curry and cinnamon oil are placed on a petri dish filled with agar then the cinnamon and curry oil would stop any growth of bacteria because curry and cinnamon are known to stop bacterial growth. Was not supported because the curry and cinnamon ultimately increased the growth of the bacteria while the controlled had barely and affect. The curry oil produced the most bacteria, even though studies have shown that curry kills more bacteria than cinnamon. It is very unlikely that this experiment will truly change the world, but more consideration could be taken. Such as, maybe just regular curry and cinnamon

The bacterial curve results in diauxic growth curve .which shows two different kinds of active growth. In the first phase of exponential growth, we are able to see that bacteria get its energy from glucose until all the glucose has been exhausted. In secondary lag phase, lactose is utilized at second stage of the growth exponential. During the glucose phase, lactose cannot be utilized because cells are unable to transport and break the disaccharide lactose. Lactose operon can be repressed even in the presence of lactose (inducer) .enzyme of lactose utilization are being synthesis after the glucose has been exhausted.

A bacterial growth curve illustrates a lag phase, a log phase, a stationary phase, and a death phase. The lag phase of the growth curve depicts no growth of the population. There is no growth because the bacteria cells are not undergoing division because the bacteria is still adjusting to the environment. The log phase depicts the period of time

The purpose of the two experiments was to determine the fundamental effects that temperature has on the growth and survival of bacteria. During the first experiment five different bacterial broth cultures of Escherichia coli, Pseudomonas fluorescens, Enterococcus faecalis, Bacillus subtilis and Bacillus stearothermophilus were individually incubated at temperatures of 5, 25, 37, 45 and 55°C for one week in an aim to distinguish the effect temperature has on growth and survival of the five different species. After one week they were observed for distinguishable changes by the turbidity showing an indication of bacterial growth, or the clarity an indication of no survival.