How Does Temperature Affect Lipase

For enzymes to be useful in a reaction, the substrate needs to bind with the enzymes active site. The active site is specific for a

The temperature can have a major impact on an enzyme. According to Campbell Biology author Reece etc. 2011 “The enzyme reaction will increase as the temperature increase with the increasing temperature….substrates collide with active sites more frequently when the molecules move rapidly.”(Reece etc 2011) Every enzyme hits its optimal temperature the reaction will be at its highest point.(Reece etc. 2011) When the

Hypothesis: I believe the rate of reaction will speed up as the temperature increases until it reaches about 37oC, which is the body temperature, where it will begin to slow down and stop reacting. I believe this will occur because enzymes have a temperature range at which they work best in and once the temperature goes out of this range the enzyme will stop working.

These results show how temperature of extreme high, or low affects enzyme activity. The highest rate of enzyme activity occurred at 37 Cº. Anything that was hotter or cold than 37 Cº slowed the reaction rate. As I thought, 100 degrees would denature the enzyme, and that was the case. The data provided shows exactly what temperatures enzymes work best, and worst. The objective was achieved as we discovered the different reaction rates under different temperatures. The results are reliable, as we know enzymes do not work well when under extreme heat or denaturation occurs. What I learned in this experiment was that enzymes don’t work well under cold temperatures because they tend to move slower. My hypothesis did not quite match, because I thought they work best at lower temperatures.

Question: How does changing enzyme concentration or temperature affect the reaction time of enzyme activity?

Hypothesis: If the temperature increases, then rate of the chemical reaction will increase as well, and if the temperature decreases, then the rate of reaction will also decrease.

The null hypothesis for the first experiment was that substrate concentration would have no effect on the reaction rate. It was hypothesized that the reaction rate would increase with rising substrate concentrations, until all active sites were bound. The null hypothesis for the second experiment was that temperature would not have an effect on reaction rates. It was hypothesized that until the enzyme is denatured, as temperature increased, so would the reaction rate.

However if the temperature exceeds the optimum temperature the enzyme becomes denatured. This is because there is too much energy causing the enzyme molecules to vibrate causing the bonds maintaining their tertiary structure to break. The enzyme unravels causing the shape of the active site to change so it can no longer fit with the substrate.

What does the changes in the amount of substrate on an enzyme’s reaction effect on?

then release the products. The principal function of enzymes is to increase the rate of the

To determine how different temperatures and how they can directly affect the outcome of enzyme activity, we will need to look at the actual temperatures used in the study as well as the specific type of enzyme used. In remembering that enzymes; Proteins speed up the rates of chemical reactions in the types of living organisms used in the lab experiment this report is based upon. With our groups’ hypotheses, including prediction: The determination factor being can a higher temperature decrease the enzyme activity? In this study we as a lab group needed to be able to form conclusions with knowing at what temperatures did we see the results?

This experiment was performed in order to determine how the condition of the environment and the specificity can affect enzymatic activity. The activity of enzyme, how efficiently the enzyme functions, is affected by general environmental factors, such as temperature and PH. It can also affected by chemicals that specifically influence that enzyme. In condition of low temperature environment, the enzymes activity will be low. As the temperature raises the rate of an enzyme-catalyzed reaction increases. The optimal temperature of an enzyme is where the temperature that the enzyme works best. Enzyme specificity is the ability of enzyme to choose exact substrate from similar molecules. Lactase is an enzyme found in small intestine that catalyzes the breakdown of Lactose (milk sugar) into the simple sugars glucose and galactose. In this experiment the enzyme Lactase and its’ substrate Lactose were used to measure the production of Glucose. This experiment will examine the specificity of an enzyme to only a specific substrate and the amount of glucose production in different temperature.

The Effect of different Temperatures on the reaction rate of Amylase Introduction: Amylases are enzymes that breaks down starch which accelerates the hydrolysis of glycosidic bonds in polysaccharides (S. Sivaramakrishnan et al., 2006). They bond with certain substrates to effectively increase the reaction rate of a chemical experiment. Most enzymes are very specific, as in they would only bond with a distinct substrate for it to cause a certain reaction. This can be described with the key and lock analogy. Only one type of key, certain amylase, will be able to open a particular lock, substrate.

Enzymes are very specific in nature, which helps them in reactions. When an enzyme recognizes its specific substrate, the

reaction rate increases. If the temperature of an enzyme gets to high the reaction rate will slow