As seen in figure 1, the amount of oxygen increased from 20.75% to 22% rapidly between 20 and 30 seconds. There was also an aggressive increase between 170 and 180 seconds from 27.1% to 28%. Otherwise, the increase was relatively stable, with slight increases and decreases throughout. On the other hand, when an acid and base (hydrochloric acid and sodium hydroxide, respectively) were added to the reactive solution, the increase was relatively stable for the acid, while inconsistent for the base, in general.
More specifically, the main spike in the acid reaction was found in between the 70 and 80 second interval. The amount of oxygen also leveled off after 160 seconds. As for the base, the largest spike in oxygen was found between 90 and 100 seconds. There were two additional spikes and plummets from 140 to 180 seconds, alternating twice. The amount of oxygen plummeted and recovered gradually from 40 to 50 seconds.
…show more content…
The materials and methods sections may be referred to in order to see how these tests were done. Overall, the cold water reaction had less oxygen gas than the warm and boiling water, which are much closer together. All increase steadily. The warm and boiling water leveled off around 130 seconds and increased at a slower rate from there. The rate of increase for warm and boiling water was highest between 10 seconds and 50 seconds. The increase of oxygen in cold water was much more constant, with a minor spike between 60 and 70 seconds.
Last but not least, the final variable tested was the concentration of the catalase enzyme, which was increased. The rate of increase of oxygen gas was stable except for one small outlier towards the
The more acidic a substance is the less oxygen it will produce when going through a chemical reaction. During the Lab “How Do Changes in pH Levels Affect Enzymes Activity”, the researcher conducted an experiment to test the effects that an acidic, neutral, and a base substance will have when combine it with hydrogen peroxide. The data table shows that HCL (acidic substance) barley produced any oxygen at all when it was combining with Hydrogen Peroxide. The pH level for HCL was 2.5; this level indicates that the substance was very acidic. When the H2O and NaOH were tested they produced more bubbles than HCL. NaoH produced a little more bubbles than HCL. The pH that NaoH produced was a 9, which is a base. H2O produced more bubbles than both substances;
Lab six requires students to observe the effects of pH and enzyme concentration on catecholase activity. Enzymes are organic catalysts that can affect the rate of a chemical reaction depending on the pH level and the concentration of the enzyme. As pH comes closer to a neutral pH the enzyme is at its greatest effectiveness. Also at the absorbance of a slope of 0.0122 the enzyme is affected greatly. The pH effect on enzymes can be tested by trying each pH level with a pH buffer of the same pH as labeled as the test tube and 1mL of potato juice, water, and catechol. This is all mixed together and put in the spectrophotometer to test how much is being absorbed at 420nm. As the effect on enzyme concentration can be tested almost the same way. This part of the exercise uses different amounts of pH 7-phosphate buffer and potato juice, and 1mL of catechol mixed together in a test tube. Each substance is put in the spectrophotometer at a wavelength set tot 420nm. The results are put down for every minute up to six minutes to see how enzyme concentration affects reaction rate. The results show that the pH 8 (0.494) affects the enzyme more than a pH of 4 (0.249), 6 (0.371), 7 (0.456), and 10 (0.126). Also the absorbance is greatest at a slope of 0.0122 with test tube C that has more effect on the reaction rate, than test tube A, B, and D.
The hypothesis is that catalase activity will increase exponentially with higher concentrations of hydrogen peroxide until all catalase active sites are filled, in which case the
Did the pH level of the blood change at all during this run? If so, how? Yes it did, when the breathing
The topic of this lab is on biochemistry.This experiment was conducted to show how cells prevent the build of hydrogen peroxide in tissues. My group consisted of Lekha, Ruth, and Jason. There were used two different concentrations of hydrogen peroxide through this experiment , 1.5% and 3%. By testing two different types it is easier to understand how the H2O2 and catalase react with one another. To do this both the yeast, which was our catalase, and H2O2 were mixed together in a beaker. Each concentration was tested out twice for more accurate results . 1.5% concentrated H2O2 had an average reaction rate of 10.5 seconds while 3% concentrated H2O2 had an average reaction rate of 7.5 seconds. From this experiment we learned that by increasing the concentration of H2O2 and chemically combining it with a catalase it will speed up the reaction. Enzymes speed up chemical reactions . The independent variable in this experiment was the concentration of the H2O2. Some key vocabulary words are Catalase, enzyme, hydrogen peroxide ( H2O2), and concentration.
4.a) Describe the effect of low (pH 2) and high (pH 12) pH levels on catalase activity.
This experiment is designed to analyze how the enzyme catalase activity is affected by the pH levels. The experiment has also been designed to outline all of the directions and the ways by which the observation can be made clearly and accurately. Yeast, will be used as the enzyme and hydrogen peroxide will be used as a substrate. This experiment will be used to determine the effects of the concentration of the hydrogen peroxide versus the rate of reaction of the enzyme catalase.
I know that with a rise of 10c the rate of reaction will be doubled in
How concentration affects the rate of reaction between Hydrochloric Acid and Sodium Thiosulphate Chemistry Coursework How Concentration Affects a Reaction Aim: The aim of this experiment is to find out how concentration affects the rate of reaction between Hydrochloric Acid and Sodium Thiosulphate. Introduction: This experiment will be carried out by drawing a cross on a piece of paper and mixing hydrochloric acid and sodium thiosulphate together to see if the cross disappears. The concentration of hydrochloric acid would change every reaction to show that concentration effects a reaction.
If the acid is made more concentrated there are more particles, which means collisions are more likely. So, the higher the concentration, the quicker the reaction time is. Temperature also affects the rate of reaction. If the temperature is increased, the particles move quicker so more collisions happen. This means the higher the temperature, the
Once this step was completed, a timer was started and observations were made. Using the timer, how long cellular respiration (this was noted by the appearance of gas in the tube) took to start was noted. Noted
The purpose of this lab report is to investigate the effect of substrate concentration on enzyme activity as tested with the enzyme catalase and the substrate hydrogen peroxide at several concentrations to produce oxygen. It was assumed that an increase in hydrogen peroxide concentration would decrease the amount of time the paper circle with the enzyme catalase present on it, sowing an increase in enzyme activity. Therefore it can be hypothesised that there would be an effect on catalase activity from the increase in hydrogen peroxide concentration measured in time for the paper circle to ride to the top of the solution.
Title: The Effect Temperature of Sodium Thiosulfate Has On The Rate of Reaction with Hydrochloric Acid
always give out or take in energy most of the time this is heat energy
was too fast to measure and so for my scale to reach above that, I