How Does The Concentration Of Substrate Affect The Reaction Rate?

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Question # 1: How does the concentration of substrate affect the reaction rate?

Hypothesis: If the concentration of a substrate increases, any time an enzyme happens upon a substrate it will be free to help that substrate react, but then when an enzyme encounters a new substrate at a slightly higher concentration it will already be working on another substrate, therefore, as we increase substrate concentration it is not going to increase as fast as the reaction rate. Argument: Substrates are reacting molecules that are acted upon in order to make what is called the product (Bio 5LA- Lab # 5, pp.1). However, this reaction would not be possible without enzymes, which are as equally if not more important than substrates because they are
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This leads me to the argument that there is a relationship between concentration and absorbance that will enable me to determine the concentration of a solution through the measurement of the color change in a reaction tube (Bio 5LA- Lab # 5, pp.3). It is evident, that the relationship between concentration, reaction rate, color change, and absorbance plays an important role in demonstrating that the reaction rate, goes hand in hand with concentration. Ultimately, their relationship will directly affect the reaction rate of an enzyme-catalyzed reaction (Maslov, Dmitri).
Experimental strategy: The experimental approach that will be taken, in order to test my hypothesis involves the use of the spectrophotometer. This machine will enable me to examine the effect of different substrate concentrations on the rate of reaction (Bio 5LA- Lab # 5, pp.3). In brief, I will have to take varying concentrations of a substrate, add an enzyme to each of tubes filled with different concentrations, and rapidly place the tube into the spectrophotometer, in order to measure the time it takes the varying substances to change color. As a result, when the color change is visible in any of the varying substrate concentrations, it will be safe to say that, the enzyme has reacted with the substrate, to form an enzyme-substrate complex. Similarly, the time it took for a color change to happen, will allow me to observe and record the rate at which each reaction
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