The authors in this article are trying to better understand how information is processed in the brain, especially the diseased brain. In order to research this information there first needs to be a precise method of spatio-temporal recording of electrical activity in larger neuronal circuits and individual neurons. The authors hypothesized that fluorescent indicators would be useful tools in recording brain activity because they would allow monitoring of a genetically defined neuronal circuit and they would not require chemical access to visualize and provide optical recording of brain activity. Since the current techniques could not meet the authors’ requirements to study neuronal activity in vivo, they sought out to create a voltage …show more content…
Next the authors made a circularly altered Clover GFP by creating termini at the amino acid positions 145 and 144 and linking its N- and C- termini using the peptide ‘GGTGGS’ between the C-terminal (K239) lysine and the original starting methionine. The authors obtained cpsfGFP-OPT145-144 (abbreviated as cpsfGFP-OPT), which is a circular permutant of a superfolder GFP variant and which contains 7 mutations that are not present in the original GFP superfolder; it was made for the use in a split GFP system. The authors then constructed circularly altered superecliptic pHlurinA227D (cpsepHluorinA227D), creating new termini at the amino acid positions 145 and linking its original N-and C- termini using the peptide GTGGSAS between the C-terminal lysine (K239) and a lysine originally near its N terminus (K4). Then authors constructed ASAP1 using the voltage-sensing domain (VSD) from the Gallus gallus voltage-sensitive phosphatase. The ASAP1 variants were made with the alternate VSDs by inserting cspfGFP-OPT following amino acid G146 (Xenopus laevis), G147 (Danio rerio), or amino acids L203 to G214 (Ciona intestinalis). The authors isolated the VSD from the VSP by cutting the C-termiminus at the same positions: T183 (G. gallus), T182 (D. rerio) or S244 (C. intestinalis). Then ASAP1 variants and Archlight Q239 were cloned between the NheI-HindIII sites of pcDNA3.1/Puro-CAG. The scientists used coexpressed sensors with a
Carol S. Dweck's article “Brainology – Transforming Student's Motivation to Learn” offers insights about student's mentality at school and why some students are better off than others. The article claims directly that students generally have two mindsets when it comes to learning; one is “fixed mindset,” a negative trait, and the other is “growth mindset,” a positive trait. Both of these traits contradict each other in terms of meaning. These two mindsets impact students on whether or not they will be successful on their academic road. In “Brainology – Transforming Student's Motivation to Learn,” Dweck explains how these two traits influence the outcome of having one of these two mindsets through
The vital components and techniques of gene cloning are as follows, the DNA sequence that contains the desired gene (EZH2) is amplified by Polymerase chain reaction. PCR was established by Kary Mullis in 1985, popularly known to amplify target sequences of DNA (EZH2) to a billion fold in several hours using thermophilic polymerases (Taq) ,primers and other cofactors (Sambrook and Russell, 2001). Three crucial steps are involved which are Denaturation (at 95°), Annealing of the forward and reverse primers (55-65°) and lastly primer extension (at 72°). After amplification the desired sequence is integrated into the circular vector (pbluescript) forming the recombinant molecule. For the compatibility of the insert and vector, both were digested with (EcoR1) so the same cohesive ends are generated in both, making it easier to ligate. EcoR1 is a restriction enzyme that belongs to the type II endonuclease class which cuts within dsDNA at its recognition site “GAATTC” (Clark 2010; Sambrook and Russell, 2001).
When it comes to the topic of having a growth mindset, most of us will readily agree that students who are praised are motivated to learn. Where this agreement usually ends, however, is on the question of how they are praised. Whereas some are convinced that praising students for their intelligence will motivate them to learn, others maintain that encouraging them for their efforts has a better impact on their motivation.
Green fluorescent protein (GFP) comes from the jellyfish Aequorea Victoria is rare proteins with high fluoresce and absorbance. The purpose this experiments is to purify and express a His2-tagged recombinant from of GFP (rGFP) from the E. coli strain BL21(DE3)< pRSETA-GFPUV > through a series of experiments by using Ni+2 agarose affinity chromatography technology. The GFPuv gene (UV-optimized GFP) was over expressed in the E. Coil strain BL21 (DE3) (pLysS) as an n-terminal His6/Xpress epitope tagged bind protein. Then using Ni2+ Agarose affinity chromatography to obtain purification of the crude extract. Then observe under the long wavelength UV light, the activity of the rGFP in the column fraction. Bradford assay was performed to obtain the total protein amount. When calculating the
The brain stem or the medulla is the closest to the spinal cord. The brainstem is responsible for most of the human bodily functions. The brain stem is critical for human survival and normal human functions. The brain stem is also responsible for information of pain and touch from the head and neck; it is also responsible for the inputs from the face and the mouth. Reticular formation is a group of neurons, nuclei, that are important for sleep and wakefulness. In reticular formation there is nuclei that are a major source of neurotransmitter serotonin. Serotonin is important for regulating mood and activities.
I have always had a passion and interest in working with the unknown. When I was in high school, I always made sure I was taking science classes that interested me, and would help me decide on what I wanted to major in college. I chose neuroscience as my major because it is a subject that continues to fascinate me. Neuroscience is the study of how the nervous system develops, its structure, and what it does. I want to focus on the brain and its impact on behavior and cognitive functions. I want to go into clinical neuroscience (looking at the disorders of the nervous system) or cognitive neuroscience, which studies the higher cognitive functions and underlying neural bases. With a neuroscience major, I would like to pursue a career in clinical research, do research for the National Institute of Health, work for the CDC and specialize in neurological disease, and/or run a clinical research project in another country. I want to pursue a career in one of these areas because I want to dedicate my knowledge, skills, and time to helping people and the world of science. I want my work to make a positive impact on society and be beneficial for the forthcoming generations. I want to help people and discover new things that will help those in need. I am motivated every day to continue working hard by realizing there are still more things to be discovered and that it could be done by me.
Dementia is an umbrella term for brain disorders that affect memory, thinking and communication. In the US alone about 5.4 million people have been diagnosed with a form of dementia of which 96% are individuals over the age of 65.
Taking a journey with your mind through the stories of reading a novel is a positive enhancement to proper brain function. In reference to the study of proper brain function and how reading a novel can improve the reader’s connectivity on a level of higher brain function; make myself think on my past neglect of not picking up a fiction book and exploring a novel for myself. Reading is the key to unlocking untapped potential in imagination with reading a book that challenges you to become one with the Arthur. The only way to truly free your mind and push the boundaries of proper brain function is to become an enthusiasm of reading fiction books.
After listening to the podcast of Hidden Brain podcast, “The Edge of Gender: Nature, Nurture, And Our Evolving Debates About Gender” (October 9, 2017) I realized that biologically we are different because of our chromosomes and genes but even thought that makes us biologically different there shouldn’t be issues on whom we decide to be. A quote from Hidden Brain states, “girls are exposed to unusually high levels of testosterone in the womb. And what we see when they are born is, they will gravitate towards male-typical toys, and this is even if their parents give them more praise for playing with female-typical toys” To true this is a key point biologically, but that doesn’t mean that it’s all-true because as we grow up we are influenced from
Carol dweck's article brainology speaks about christopher situation and aspects regarding his fixed and growth mindset. By providing example from the text and carol dweck's article, this essay will further support his growth or fixed mindset, and thoroughly analyze the article itself.
The second method was to purify plasmid DNA from the E. coli culture and physcically analyze the DNA by digesting the plasmid with specific restriction endonucleases. The restriction endocucleases used were Bam HI (B), EcoRV (E), and BgIII (G). These restriction endonucleases recognize specific short DNA squences. These cut plasmids were then placed in a 1% aragrose gel to determine the band sizes. The expected phenotype for Pglo is that there will be floursenscens light produced on the LB + amp + Arab plate, where as for PFG the expected phenotype is that there will be flourcent phenotype only in LB+ Amp + IPIV. For the physical analysis Pglo will produce fragment sizes in restriction enzyme (E) 5371bp long. Enzyme (B). fragment sized 625bp, 220bp, and 4,526bp in restriction enzyme (B). Pfg will produce 2 fragments in restriction enzyme (E), one will be around 3,855bp long, and the other one will be 2,506bp long. In restriction enzyme (B) there will be only one band about 6361bp long. From restriction enzyme (G) there should be one bad around 6361bp
Psychologists constantly offer new testable hypothesis to expand our knowledge on human behavior. This paper will include an analysis of a podcast and a research article. From this analysis, a hypothesis on birth order will be offered toward the end of this paper.
In this essay I will be writing about the research I’ve done on the brain disorder Alzheimer’s. I will discuss all aspects of the disease, from how it first gets diagnosed to how it affects your body and how it may end with your eventual demise. I will also discuss my first-hand experience with my grandma and her battle with Alzheimer’s. I will explain the happenings inside of the brain in an Alzheimer’s brain vs the regular healthy human brain.
The oscilloscope was used to determine that electrode placement was sufficient to record neural activity. This was determined by looking for a recording with minimal background noise and clear spontaneous extracellular action potentials (EAPs) . Criteria for an EAP included the presence of a clear bi- or triphasic waveform amplitude peak above 10 mV.
One field of study in the realm of biology that still leaves much to be discovered is neuroscience, more specifically, the neurological pathways and signaling within the brain. While there have been many developments and breakthroughs in identifying areas of activity and neural pathways, there are still many obstacles to overcome and discoveries to be made. The authors of the journal High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor sought to overcome one such obstacle. They understood the potential for fluorescent proteins to be used for visualizing the firing of action potentials throughout neuronal pathways of the brain, but realized that such proteins were unable to accurately record higher frequency signaling across synapses where there was frequent action potential activity. Within this journal, they describe the methods used to produce a new fluorescent protein, accelerated sensor of action potential 1(ASAP1), and the benefits that it can provide to the field of visualizing the activity of action potentials as they propagate through synapses in the brain. They hypothesized that ASAP1 should be able to give definitive fluorescent readings for the activity of action potentials propagating through the brain, even in areas with high frequency activity that have not been measurable before.