How To Make 10 Agar Plates

I put one test tube for each control, substrate, and enzyme in the 4° C (ice bath), 23° C (room temperature), 37° C (body temperature), and 60° C (water bath). Add the inhibitor that was used and what it was used for.

6. The disks in the 0.00% solution were transferred to an agar plate held next to the blue flame using the sterilized tweezers. Excess disinfectant was removed from the disks by wiping on the side of the well of the spotting tile. When the 5 disks were positioned (refer to Figure 1 below) the lid was replaced and sticky taped down. A label was added indicating the concentration of disinfectant.

This test is used to detect the hemolytic activity in the bacteria. A darkish green color on the media around the bacteria would represent incomplete hemolysis. A transparent media around the bacteria colony represents complete lysis of the red blood cells. If no change is observed around the bacteria colony then the bacteria is non-hemolytic. For my

C. Pour about one quarter of the first unknown packet into the first cup and add

The dialysis tubing will be clamped at one end in order to fill it and then clamped at the other end to seal the filled bag. If the bag is not soft and floppy, the experiment will not work. Blot a bag with a paper towel to absorb the moisture and weigh it, if this blotting process is not done it could interfere with the weight readings creating inaccurate information. After the bags of the solutions are prepared, they will be placed into five different beakers with different solutions. Beakers 1-4 will be filled with tap water and the fifth beaker is filled with 40% sucrose and water. Fill each beaker with just enough water or solution so that the bag is covered and place the bags in the beakers simultaneously and record each time. Every 10 min the bags are to be taken out, blotted, and weighed again before returning them back into their respective beaker for another 10 min. The process is repeated until you have reached 90 min. The weights should be recorded in grams (g).

Read in your lab manual about the following agar mediums: Blood Agar (pg 168), EMB Agar (pg 170), Mannitol Salt Agar (MSA)(pg 172) ), MacConkey Agar (pg 174), and PEA Agar (pg 176) to answer the following:

Using the graduated cylinder, measure 20mLs of the stock sucrose solution and 180mL of water to create a 3% sucrose solution and place it into the 250mL beaker (beaker #2). Place bags #1‐3 (red, blue, yellow) into beaker 2 and bag #4 (green) into beaker 1. Allow the bags to sit for one hour. After allowing the bags to sit for one hour, remove them from the beakers carefully open the bags, noting that often times the tops may need to be cut as they tend to dry out. Measure the solution volumes of each dialysis bag using the empty 250 ml beaker.

The first experiment begun by filling a 600-ml beaker, almost to the top, with water. Next, a 10-ml graduated cylinder was filled to the top with water. Once water was added to the beaker and graduated cylinder, a thumb was placed over the top of the graduated cylinder. This would ensure that no water was let out and no bubbles were let into the graduated cylinder. Next, it was turned upside down and fully submerged into the beaker. Then, a U-shaped glass tube was attained. The short end of the glass tube was placed into the beaker with the tip inside of the graduated cylinder. Next, a 50-ml Erlenmeyer flask was received. After, 10-ml of substrate concentration and 10-ml of catalase/buffer solution were placed into the flask. A rubber stopper was then placed on the opening of the flask. After adding these, the flask was held at the neck and spun softly

Start the LoggerPro software. Perform a trial run Enzyme Assay. Pipet 4 ml of the pH 7 buffer at your lab station into 8 test tubes. Add 2 ml turnip extract to each test tube. Now pour 0.2 ml of silver nitrate into 2 test tubes. Repeat this step 3 more times adding silver nitrate in increments of 0.2 ml (i.e., 0.4ml of silver nitrate next, then 0.6 ml of silver nitrate, etc.), so that each of your test tubes has a different percent solution of silver nitrate.

To begin(control data and experiment data), fill the solution container with designated amount of live yeast and 10 mL of hydrogen peroxide. Quickly after mixing solutions together, cap the container with the cork and straw and cover the open end of the straw. Have one member of the lab begin the timer. Keep the open end of the straw covered to submerge the solution container under water in the tub and let the open end of the straw be placed inside the open end of the graduated

30mg guaiacol is weighed and dissolved in 50mL methanol as assay. 10mL of this assay is diluted to 50mL with methanol in order to be used as standard solution.

Before the streak technique was performed, the sample was “vortex” to mix solution, then a loop was sterilized with a flame. The loop was then used to aseptically inoculate a sample from the vial and a lawn was created on the plates, the loop was again sterilized. From the lawn, about 6-8 directional streaks were made using the edge of the loop; then the loop was flamed. The plate was turned slightly to make

For this experiment we utilized a variety of materials. We first utilized a sharpie pen to write down the locations of our experiments and our names among other information on seven different Agar plates. We then used seven different containers of sterilized water to make sure there was no contamination between different samples. We also utilized seven different disposable swabs for this exact same reason. We also used seven packages of Para-film in order to seal the plates. Lastly we made use of a freezer in order to keep our plates at the proper temperature for bacteria incubation and growth. These are all the physical materials we made use of while conducting our own experiment.

After the water was boiled, a black tea bag was then placed into one of the beakers and steeped for 4 minutes. This time was monitored using a stopwatch. After the tea had been steeped, 6 discs were dipped into the tea solution and then placed in the appropriate agar plate (plate #3). The discs were fairly equal distances apart. The same procedure was done with the other beaker of boiled water, the antibiotic, and the different percentages of tannic acid. A total of 6 discs were placed on each plate (ex: 3 dipped in boiled water, 3 in antibiotic in plate #1).

1. Number four clean test tubes 1-4. 2. Place 1 ml (or 20 drops) of the following solutions into each tube: water, egg albumin, starch, and chicken broth. 3. Add 3 drops of Buiret reagent to each tube and gently shake