How To Write A Pglo Transformation Lab Report

The GFP gene is normally isolated from the jellyfish Aequorea victoria, allowing it to fluoresce with the absorption of ultraviolet light (Goodsell, 2003). This gene is inserted into a region of a plasmid to be regulated by an operon (Brown, 2011). In the plasmid, GFP is normally repressed but when arabinose is present, it will induce the gene to make enzymes able to digest the arabinose, and once there is no more arabinose,

13. Use a sterile plastic loop to distribute the cell suspension evenly on the plate by “skating” the loop back and forth across the LB agar plate several times.

Green fluorescent protein (GFP) comes from the jellyfish Aequorea Victoria is rare proteins with high fluoresce and absorbance. The purpose this experiments is to purify and express a His2-tagged recombinant from of GFP (rGFP) from the E. coli strain BL21(DE3)< pRSETA-GFPUV > through a series of experiments by using Ni+2 agarose affinity chromatography technology. The GFPuv gene (UV-optimized GFP) was over expressed in the E. Coil strain BL21 (DE3) (pLysS) as an n-terminal His6/Xpress epitope tagged bind protein. Then using Ni2+ Agarose affinity chromatography to obtain purification of the crude extract. Then observe under the long wavelength UV light, the activity of the rGFP in the column fraction. Bradford assay was performed to obtain the total protein amount. When calculating the

Green Fluorescent Protein, produced by the bioluminescent jellyfish Aequorea victoria, is a protein that fluoresces green under ultraviolet light. Since its discovery, properties of the protein have been improved by mutations in the gene resulting in the expansion of its spectrum, which now contains brighter variants and multiple different colors. GFP is used in a wide variety of applications and technologies. Its many different applications have contributed greatly, and continue to do so, in numerous fields of study including, but not limited to, cellular and molecular biology, microbiology, biotechnology, and medicine.

Transformation is the transfers of virulence from one cell to another, through the transferring of genetic material. It was originally postulated in 1928 through the works of Federick Griffith, a British microbiologist. Griffith observed that the mutant form, non-virulent form, of the bacteria Streptococcus Pnumoniae could be transformed into the normal, virulent form, when injected into mice along with heat killed normal forms. He concluded that somehow the information the dead virulent form had transformed the mutant form into a virulent form.

Transformation is when you give organisms new traits, like ampicillin resistance, by the process

For millions of years’ bacteria have been surviving to their environments and been at war against other microorganism. This wars have lead them to creating an unstoppable arsenal of weapons they can use to survive. Unlike virus that need a host cell to survive, bacteria can survive anywhere since they can share their DNA with each other. This allows bacteria to survive in places like radioactive waste, zero oxygen environments, and even in absolute darkness. Unlike human beings who are born with a specific genetic code, bacteria have the capability of changing that code. Bacteria have three methods of evolving themselves in order to survive the harsh is of conditions. The three methods are called horizontal gene transfer. The first is Transformation, but some pathologists call it the “Funeral Grab”.

Griffin was a scientist that showed that there was a non-living component to heritability between organisms, although he had not yet discovered what exactly was the heritable component. However, during this time period, it was a common belief that the heritable material was contained within the protein of the organism, not the DNA that is now held in the truth. In the Avery and company experiment, they expanded Griffin research to pinpoint the exact location of the heritable information by breaking down cell components until the final product allowing transformation was

A lab write up can make or break your results in the science world. Structure is very important. The article “Materials & Methods” is basically a step by step how to guide for anyone learning how to write a lab report.

Introduction Genetic transformation is known to be the conversion of a genotype into another with the addition of DNA from an external source. Frederick Griffith was the first person to use genetic transformation in 1928 with Streptococcus pneumoniae (Griffiths et al. 2000). There are three different methods that are used in genetic transformation to get the vector DNA into the nucleus of the competent cell. The methods are, projectile bombardment, electroporation, and heat shock. Heat shock is the most common method is projectile bombardment because it guarantees the mixture of the two DNA (APSnet…2014).

The pGLO plasmid is being introduced. This plasmid contains genes for GFP

Depending on the bacteria species, the manner in which transformation occurs varies. Haemophilus influenza is an example that utilises membrane-bound vesicles to capture double-stranded DNA 3. On the other hand, S. pneumoniae can uptake single-stranded DNA molecules after its cells express competency factors 3. Alternatively, scientists in laboratories transform cells, including ones that are not naturally competent, by inducing them to uptake DNA 3. These transformed cells are now known as competent cells for they have been rendered able to uptake DNA from the environment 18. This artificial transformation process is achieved by using a procedure called the heat shock method 18. This transforming method includes adding DNA to cells in the

The heat shock treatment increases the rate of successful transformation by making the bacterial plasma membrane more permeable to the plasmid solution. During the experiment performed in the Life 102 Lab we took a sample of Escherichia Coli (E. Coli) bacteria and transformed it using a gene that codes for green fluorescent protein (GFP). (Weedman 2016). The GFP originates from a bioluminescent jellyfish, and the protein is what allows them to glow. The GFP gene is only visible in the presence of arabinose.

As a result of this lab it was learned that the E.coli would only glow if the GFP or the “glow gene” was activated in the pGLO by “Ara”, the arabinose-sugar, under a UV light. The outcomes of this lab could lead scientist in the right direction towards finding out how to successfully implant the “desirable traits” of one organism into another organism like the pGLO Transformation Lab demonstrated. With being able to take the desirable traits of one organism would be extremely beneficial to human life and using this lab as a supportive back bone to future experiments like taking the venom of Androctonus Australis, one of the most deadly scorpions in the world, and genetically transforming it with cabbage (Handley, Andrew 2013). The experiment deemed the cabbage safe for human consumption because the toxin “AaIT” found in the scorpion's venom only kills insects, therefore the cabbage is its own insect repellent which could cut down on harmful pesticides by taking the “desirable trait” from the scorpion and implanting that trait into the cabbage like what was demonstrated in the pGLO Transformation Lab. The pGLO Transformation Lab demonstrated how organisms can be genetically transformed, what factors affect transformation, and this lab hopefully prompts for more experiments with genetic engineering and transformation for the best outcomes, and maybe the new generation will figure out to increase anything beneficial to human survival by using genetic engineering and transformation just like what was demonstrated in the pGLO Transformation

Bacterial transformation is the process of moving genes from a living thing to another with the help of a plasmid.The plasmid is able to help replicate the chromosomes by themselves; laboratories use these to aid in gene multiplication. Bacterial transformation is relevant in everyday lives due to the fact that almost all plasmids carry a bacterial origin of replication and an antibiotic resistance gene(“Addgene: Protocol - How to Do a Bacterial