preview

Human Ipsc Line, 201b7 Clone Essay

Decent Essays

1. The source of the iPSC’s in this case study are human iPSC line “201B7 clone”. They are dermal fibroblast tissues that in previous studies have been shown to have low tumorigenicity (meaning they have a low ability to produce tumors) after being used in transplantation therapy.
2. The iPSC’s were different from the host cells because a piggyBac vector was introduced into the hiPSC. A piggyBac vector is a genetic element that literally does what it sounds like, gives a piggyback between vectors and chromosomes, and cuts genes, carries them, and pastes them into a new position. This specific piggyBac vector used to distinguish the host cells from the iPSC’s stably expressed GFP (green fluorescent protein) gene under a ubiquitous promoter. The promoter was EF1 (elongation factor used to push gene expression in vitro and in vivo). The authors knew their transplantations of iPSC’s were successful because once the cells were introduced they observed continuous GFP fluorescence. The GFP fluorescence was observed even after differentiation of neural-linage.
3. iPSC’s were differentiated into neural stem cells by using SMAD-pathway inhibition. Green fluorescent proteins were labeled iPSC, and then a “serum- free floating culture of embryoid body-like aggregates method” was combined with the SMAD- pathway. These methods stimulated the LIF/BMP signaling. Leukemia inhibitory factor and bone morphogenic protein both promote neural cell differentiation into GFAP (Glial

Get Access