Hydrogen Ions Lab

Satisfactory Essays
The concentration of hydrogen ions (H+) is measured by pH. The higher the H+ concentration, the lower the value of pH, meaning there is a large concentration of H+ in acidic solutions.
H+ ions are positively charged that they are attracted to negative ions. Many ionic and hydrogen bonds keep the enzymes tertiary structure in place to maintain the enzyme’s active site in the right shape. The ionic and hydrogen ions occur due to the attraction between groups on the amino acids that charged oppositely that make the enzyme protein. Therefore, changes in the concentration of H+ will change the tertiary structure of the enzyme, so the active site will be changed as well, and therefore can change the enzyme controlled reaction rate. According to the
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The charges around the active site will be changed as increasing the concentration of H ions; because there would more H+ attracted to any oppositely charged groups in the active side and congregate around them. So, that would intervene with the substrate binding to the active site and as a result that will change the enzyme-controlled reaction rate.
Each enzyme has an optimum pH where the rate of activity of the enzyme is at its highest. This pH optimum of an enzyme signifies that H+ ions concentration is the right one to give the enzyme’s tertiary structure its right shape, which is in charge of carrying the enzyme’s active site in the shape that is complementary to the substrate, so the reactions could be catalysed.
So a slight change of pH would drop the reaction rate because the enzyme’s shape is disrupted, thus the enzyme’s active site would change. But the change in the values of pH would not denature an enzyme. The bonds would be interfered because of pH change, however they can reform when the pH returns to the optimum point. The enzyme’s denaturisation only happens if there is an extreme alteration of pH (the enzyme at too low or high
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