Hydrolysis Report

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Hydrolysis Report
Bile Esculin Hydrolysis, Starch Hydrolysis,
UreaHydrolysis, Casein Hydrolysis

Hydrolysis Report

Introduction

Hydrolysis is a chemical reaction that uses water to split complex molecules. The water molecule H2O is split in the mechanism of hydrolysis, hydrogen cations and hydroxide anions. When the enzyme catalyzes its reaction inside the cell, it is referred to as intracellular hydrolases. When the enzymes secreted from the organism to catalyze reactions outside the cell, it is referred to as exoenzymes or extracellular hydrolases. Four hydrolysis reactions are discussed in the report, bile esculin hydrolysis, starch hydrolysis, urea hydrolysis and casein hydrolysis. Bile esculin hydrolysis determines whether an
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Bile esculin agar is both a selective and differential medium. It contains primarily esculin, bile salts and ferric citrate. Bile salts, the selective agent and inhibitor, inhibit the most Gram-positive cocci other than both Enterococcus and group D Streptococcus to hydrolyze esculin in the bile salts. Esculin, a hydrolytic substrate, is a glycoside of esculetin and glucose. Esculin can be easily hydrolyzed under acidic condition via
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