Hypotensive Effect of Aqueous Extract of the Leaves of Phyllanthus Amarus Schum and Thonn (Euphorbiaceae)
3230 Words13 Pages
Acta Poloniae Pharmaceutica ñ Drug Research, Vol. 64 No. 6 pp. 547ñ552, 2007
ISSN 0001-6837 Polish Pharmaceutical Society
HYPOTENSIVE EFFECT OF AQUEOUS EXTRACT OF THE LEAVES OF PHYLLANTHUS AMARUS SCHUM AND THONN (EUPHORBIACEAE).
*FABIAN C. AMAECHINA and ERIC K. OMOGBAI Department of Pharmacology and Toxicology, University of Benin, Benin City, Nigeria
Abstract: The plant of Phyllanthus amarus is used as diuretic and to lower blood pressure in traditional medicine practice. The effect of the aqueous extract of the leaves of Phyllanthus amarus on blood pressure was evaluated in normotensive male rabbits. Intravenously administered aqueous doses (5 mg to 80 mg/kg) of the extract to anaesthesized normotensive male rabbits produced a…show more content… The infusion of the aerial parts has been reported to be used to manage non-insulin dependent diabetes mellitus, for the relief of stomach ache and to treat dysentery (5). The plant extract has also been reported to have antitumor and anticarcinogenic activity (6). As a con-
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FABIAN C. AMAECHINA and ERIC K. OMOGBAI
Effect of extract on blood pressure and heart rate Each rabbit was anaesthetized with pentobarbital at the dose of 40 mg/kg, administered intravenously through the marginal ear vein, previously canulated with 21-G butterfly canula. The trachea was canulated with a plastic canula to ease respiration. The vagus nerve was located and separated from the carotid artery, which was cleared of connective tissues and canulated with a plastic canula connected to a pressure transducer via a three-way tap. The pressure transducer transmitted the blood pressure to Ugo Basile twin channel recorder (model 7090), which recorded the blood pressure and heart rate. The channel recorder was always calibrated before and after each experiment, using a mercury sphygmomanometer. When the animal had stabilized and the blood pressure and heart rate had remained constant, the extract was administered in graded doses of 5 mg/kg, 10 mg/kg, 20 mg/kg, 40 mg/kg and 80 mg/kg. The effects of the graded doses of the extract on blood pressure