Identification And Characterization Of Soybean Veins

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Egyptian journal of Virology (EJV).,(13):1.13pp.
Identification and Characterization of Soybean vein necrosis virus (SVNV): A newly Isolated Thrips-Borne Tospovirus in
A.S. Abd El-Wahab 1 and Manal A. El-Shazly 2
Faculty of Agriculture, Economic Entomology Department, Cairo University, Giza, Egypt,
Virus and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Res. Center (ARC), Giza, Egypt.
A virus isolate infecting soybean (Glycine max L.) with characteristic symptoms start as yellowish patches (chlorosis) along the leaf,s main vein and sometimes between the veins, which turn into necrotic brown or reddishbrown areas was collected from the Experimental farm of Faculty of
Agriculture, Cairo University ,Giza Governorate during May 2014/2015. To identify the causal virus, the plants were tested by enzyme-linked immunesorbent assay using antibodies against different Tospovirus i,e. Tomato spotted wilt virus (TSWV), Iris yellow spot virus (IYSV) and Soybean vein necrosis virus (SVNV). Preliminary results revealed the presence of Soybean vein necrosis virus (SVNV) in tested soybean plants by double antibody sandwich (DAS-ELISA) using SVNV specific antiserum and by tissue and dot blotting immuneo-binding assay. SVNV was able to infect only 11 plant species from 28 tested by mechanical inoculation. The virus was transmitted by mechanical means and by phytophagous thrips species; the most efficient vector was
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