General Unknown Introduction: The general unknown lab combines methods and ideas around biochemical and physiological activities of microorganisms for the identification of unknown pure culture samples of gram positive and negative bacteria. The purpose of using biochemical and physiological aspects of bacteria for testing and identification is used in everything from choosing an effective treatment for microbial-causing diseases to understanding the chemical reactions that occur within our
The purpose of this experiment was to identify two species of bacteria from an unknown mixed sample out of a possible twelve strains. With the intentions to correctly identify the unknown bacterium, a plethora of test were executed and documented, yielding the appropriate results for that particular sample. In this experiment, many methods were critical in the identification of the unknown. A dichotomous key, streaking and staining techniques, microscopy, differential and selective media, colony
The objective of the experiment was to identify the unknown salt that was given. The unknown salt contained an anion and a cation labelled, 38A. A series of preliminary tests were first conducted in order to help choose which confirmation tests should be used to identify the compound. The preliminary tests included pH and solubility, and the behaviour of the salts with concentrated sulfuric acid. The compound was found to have a slightly acidic pH, around 6.9 while being soluble in water. The compound
take place for further identification of the unknown organisms. The grain stain technique determined that unknown four was gram negative so the catalase test was further used where a small sample of the bacterium was added to a slide and a small drop of hydrogen peroxide was added directly to the unknown bacterium where no bubble formed. Further testing was also administered to unknown four such as citrate, urease, gelatinase and motility. With the citrate test a small sample taken from the agar plate
The unknown compound was solid at room temperature and was white. It was soluble in water, as indicated by Table 1. Of the list of possible known compounds, the unknown could not be CaCO3 or MgCO3 because neither one of those compounds are soluble in water, but it could have been any salt as the compound is ionic. From there, a conductivity test proved that the unknown compound conducted 1.1 volts of electricity, meaning the compound contained both positively and negatively charged ions that move
Abstract: The objective of the investigation was to perform multiple experiments that would lead to the identification of an unknown bacteria. This bacteria was causing a urinary tract infection on a patient. A sample of the bacteria causing the infection was received and analyzed. The first step was to isolate the bacteria to insure that sample was free of contamination. A MAC plate, a PEA plate, along with a Nutrient agar plate where used to produce pure colonies by using the quadrant streak
The identification of bacteria is a fundamental objective of microbiologists. It is essential to distinguish specific bacterial properties to understand the environment, physiology and disease. As new bacterial species emerge and existing ones evolve into different strains, it is imperative that microbiologists continue to isolate bacteria from the field, identify their findings and research newly discovered forms. Their discoveries can then be used to evaluate the types of microbial life that may
Lab 2 Report- Identification of Substances by Physical Properties The purpose of this lab was to see how certain substances; Naphthalene, Toulene, and and 2 unknowns (one liquid, one solid) react with 3 different solvents. To identify the two unknowns, testing needs to be done to find the density of both the liquid and the solid, determine the melting point of the solid, and the boiling point of the liquid. The Physical Properties of Pure Substances Table can then be used to compare the observed
compared in CODIS. CODIS is a databank of DNA samples. CODIS stands for combined DNA index system, it was created by the FBI and is maintained by them as well. The purpose of this databank of DNA is to help in investigations with linking samples to a suspect and to help a forensic lab match samples faster than before it was created. A CODIS search only allows access to certain pieces of information like; an identification to a lab, a sample identification number, the specific DNA characteristics or
An unknown sample is identified using the relationship between the mass of the product and mass of the reactant in a balanced chemical equation using the involved species molar mass. THEORY The relationship between mass of the product and mass of the reactant taken directly from the balanced chemical equation and molar masses of the involved species is used in this experiment to find the mass of the unknown sample. In this experiment the unknown sample is found out ,which will be on of the following