Identification Of An Unknown Sample Essay

There are many differents ways to identify a bacterial unknown and many different situations where identification would be beneficial. One way to identify bacterial unknowns is to perform biochemical tests. In this experiment multiple biochemical tests were done, by performing these tests on the bacterial unknown received the two different bacteria were then identified. The citrate test is done to test the ability of organisms to use citrate as a carbon source. This test uses Simmons citrate agar, the agar contains sodium citrate as the only carbon source and has bromothymol blue as the pH indicator. The organisms that use citrate as a carbon source use the enzyme to transport the citrate into the cell. The cells converts ammonium dihydrogen

In everyday life we run into millions of microbes. Some of the bacteria is capable of making humans sick. The study of microbes has become very important subject to help stop potential pandemics and ensure proper hygiene. Anthony van Leeuwenhoek was the first scientist who discovered bacteria. In my study of microbiology, I got to choose an unknown bacteria to identify. It is important to start off finding the family of the unknown bacteria and then eliminating one by one the species to know which bacteria it is. By knowing the family, a scientist can save themselves a lot of time by knowing which tests need to be performed for the elimination process. This might seem like a waste of time to one but being able to correctly identify bacteria

For many years the identification of microorganisms has been important in the world of medicine. It is essential or correct disease diagnosis in patients and for proper treatment. Knowing the correct identity and characteristics of microorganism is crucial when disease outbreaks occur in populations, also knowing how humans can benefit from microorganisms is important; many can be used in making certain foods or antibiotics.

Often scientists work with bacteria that do not come in a labeled test tube— for example, bacterial samples taken from infected human tissue or from the soil—and the scientist must then identify the unknown microorganism in order to understand what behavior to expect from the organism, for example, a certain type of infection or antibiotic resistance. However, because of the relatively few forms of bacteria compared to animals and because of the lack of bacterial fossil records due to their asexually reproductive nature, the taxonomy used to classify animals cannot be applied to bacteria (Brown 275). In order to classify unknown bacteria, a variety of physiological and metabolic tests are available to narrow a sample down from the fathomless number of possibilities into a more manageable range. Once these tests have been performed, the researcher can consult Bergey’s Manual of Determinative Bacteriology, a systematically arranged and continually updated collection of all known bacteria based on their structure, metabolism, and other attributes.

There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that I have been learned so far in the microbiology laboratory class for the identification of an unknown bacterium.

Dissolved oxygen is oxygen that is trapped in a fluid, such as water. Since many living organism requires oxygen to survive, it is a necessary component of water systems such as streams, lakes and rivers in order to support aquatic life. The dissolved oxygen is measured in units of parts per million (ppm). Examine the data in Table 4 showing the amount of dissolved oxygen present and the number of fish observed in the body of water the sample was taken from; finally, answer the questions below.

There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of unknown bacteria. The identification process can be completed with a series of deferential stains and biochemical tests. Creating a dichotomous key helps to limit the amount of biochemical tests done on an unknown organism and by observation

Bacteria are ubiquitous; they can be found on the skin, in the soil, and inside the body. Because of the very nature of this ubiquity, it is important to be able to determine between different strains of bacteria. An example of this is determining the causative agent for a disease so that the patient will be treated with the appropriate antibiotics. It may be important to determine the bacteria in a certain region, because like with enteric bacteria, it is normal to find them in the digestive tract as they are in a symbiotic relationship with our bodies in this area; however, they also cause opportunistic infections in places outside of the digestive tract to our detriment, such as with a urinary tract infection. Some strains of bacteria are common to nosocomial infections, and identifying these bacteria as such helps create the guidelines for healthcare workers in antiseptic technique. All of the morphology and characteristics of each strain of bacteria help us to better understand the role of bacteria in the body as well as helps us understand how they can cause illness, and what treatment regimen to set in place. In lab this semester, a sample of unknown

The identification of unknown organisms carries important ramifications that can be applied to many real world scenarios. In keeping with quality assurance beverages, food, cosmetics, and other products are frequently inspected for contaminants resulting from a presence of pathogenic bacteria. In medicine, a physician’s diagnosis and consequent treatment is largely determined from samples collected from infection sites that have been analyzed using microbial tests.

survived and someone that they cared about had to die. This is one of the

The purpose of the bacterial unknown independent study experiment completed throughout the course of this lab was to determine the identity of an unknown bacterial species. The unknown bacteria sample was chosen from numerous samples provided by the instructor. The starting unknown sample, unknown #15 was a mixed bacterial culture and a broad approach taken to identify the sample. Various biochemical tests were completed to identify the bacterial species along with the use of databases such as Gideon and Bergey’s to compare the test results of known bacteria to the results of the unknown sample. Information was gathered from the other sources and databases and phenotypic testing completed and the results compared to the database results. Aseptic

For this experiment we analyze the percent water in a crystalline hydrate. By examining the percentage we then are able to identify the changes in compound and compare them to that of unknown possibilities. In order to identify what hydrate we’re experimenting with, it must first be weighted using an analytical balance and record its initial state. To begin the heating process, the hydrate is moved back and forth over a flame at a 45 degree angle in order to remove any water within the hydrate. Thus it becomes a anhydrous residue after heat is applied. In order to achieve a constant weight of 0.002g or less, the anhydrous needs to be heated several times in order to eliminate most of the water contained. By calculating the the initial and final stage of the hydrate, the percent water will be determined. Finally, by comparing the mass of water lost from the unknown hydrate, the compound can be referenced from a list of unknowns.

Random division produces grape like clusters called staphylo. Bacilli divide only in one plane, but they can be side by side or end to end. Gram positive bacteria have a thick peptidoglycan layer and is located external to the cell membrane. Helps in cell maintenance and contributes to acidic polysaccharides such as teichoic acid and lipoteichoic acid.

This study was done to determine unknown microorganisms that were retrieved from pond water in Rocklin, California. If the study involves identifying microorganisms from water, various amount of test are done to find what type of bacterias are in water. The most common bacteria in the genera Eschericha, Pseudomonas, Acinteobacter, and Aeromons are amongst the top bacteria found in water. As a result, one or more of these microorganism will be found in the pond water collected. It is important to specify one of these bacteria in the water sample as it is potentially harmful. Some strains of Eschericha Coli are known to cause “serve gastrointestinal infection, dysentry and even death in susceptible individuals”(Wilson, 2015). Most importantly, it is important to find these species that can cause danger to humans. So, they can be treated accordantly with the right antibiotics if they presented any of the side effects.

Shape, internal composition and respiration mode are three ways bacterium are classified. Differences in internal composition varies by the amount, thickness, of peptidoglycan in bacteria cell walls. Gram negative cells have a thin layer of peptidoglycan within the membrane layers, whereas gram positive have a thick layer on the