the Unknown Project is to help microbiology students learn to identify an unknown bacterium in a mixed culture by performing different types of tests in a lab. The student must be familiar with the tests and should be able to analyze and interpret the results. Based on the results attained, an organism may be proposed. But what is a mixed culture? A mixed culture contains either two or more different types of identified microorganisms in a container, like a test tube for example. The culture may
of an Unknown Bacteria Abstract This laboratory experiment’s objective was to take a pure culture and isolate it from a mixed culture. The other part of the objective was to ascertain what species of bacteria that the pure culture was. The hypothesis made stated that so long as lab protocol was followed, the unidentified culture would be positively recognized/identified. An isolated pure colony of the unknown culture was obtained using the quadrant streak plate method. Afterward, the culture was
lab was to identify unknown bacteria cultures using various differential tests, and my unknown bacteria is #17. The identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Whether the tests performed identified specific enzymatic reactions or metabolic pathways, each was used in a way to help recognize those specifics and identify the unknown cultures. The differential tests used to identify the unknown
isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class. Each test performed, provided us with some key information about the unknown microbes in question and how the bacteria function. Materials and Methods Over a two week period, eight prepared types of test media were provided to identify the assigned unknown mixed cultures. Not all
this project was to identify the identities of two unknown bacteria in a mixed broth culture by using several separation methods. To separate the organisms, a four-way streak plate technique was used to isolate the two unknown bacteria into separate visible colonies. Then after each colony were clearly isolated; the two unknowns were processed through Gram staining test to determine the Gram stain and morphology. After Gram staining, a carbohydrate test was performed on each unknown to determine if it
Section I- Introduction Microbiology is the biological study of organisms that are too small to be seen with the naked eye (1). Microbiology includes examining a variety of different microbes such as bacteria, archaea, protozoa, fungi and viruses (1). By examining these microbes, their relationship with people and the environment they grow in, is better understood. These examinations of microbes have played a major role in comprehending how microbes can be helpful and harmful, and how
The purpose of this experiment was to identify two species of bacteria from an unknown mixed sample out of a possible twelve strains. With the intentions to correctly identify the unknown bacterium, a plethora of test were executed and documented, yielding the appropriate results for that particular sample. In this experiment, many methods were critical in the identification of the unknown. A dichotomous key, streaking and staining techniques, microscopy, differential and selective media, colony
Introduction Whenever there is an unknown disease caused by microorganisms, tests are usually made in order to identify the organism causing the disease. There are several tests that need to be made and they include tests such as performing a gram stain, streaking a plate to isolate colonies, inoculating a broth culture, inoculating API strip, and performing oxidase and catalase tests. Having knowledge on how to identify these tests are of high importance in the medical field so it would be to the
Abstract The assigned unknown in tube #3 was classified as a gram positive bacteria shaped as Cocci Chains. Enterococcaceae is the family of the streptococcus faecalis. The gram positive bacteria included tests such as the Catalase, Coagulase, Mannitol Salt Agar (MSA), Streptococcus Faecalis Medium (SFM), and the Bile Esculin test. The purpose of these tests was to distinguish which unknowns were positive vs negative as well as to determine the morphology or shape of the unknown bacterias. Introduction
Laboratory Report: Isolating & Identifying Bacteria Introduction: Through the conduction of numerous experiments, the identity of two bacterial isolates was determined. The tested specimen was an unknown sample of a mixed culture of two different species of bacteria. The first step that was taken was obtaining a pure culture of each species of bacteria by isolating one species from the other. Once isolation was complete, the isolated cultures were tested using procedures that had been performed during