Identifying Macromolecules Using Biochemical Tests

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Identifying Macromolecules Using Biochemical Tests Introduction Lipids, nucleic acids, proteins, and carbohydrates are macromolecules, which also makes them organic compounds. Any macromolecule made of subunits in a living organism is an organic molecule. Carbohydrates are molecules of C, H, and O in 1:2:1 ratio, proteins are composed of amino acids, lipids are nonpolar molecules mostly C-H bonds and are usually hydrophobic, and nucleic acids are made of nucleic subunits, most identifiable as DNA/RNA. In today’s experiment we identify the presence of these macromolecules through a series of biochemical test and controlled experiments, which has a control used for comparison. Each of these molecules has a reagent that will bind with it and cause a reaction. Both the positive control, which contains the variable that is being tested for, and the negative control, or the solvent will be able to be identified. Purpose The purpose of this lab was to identify the presence of macromolecules in different solutions using different biochemical test and to understand what it means to have a control and the importance thereof. Materials Test tubes Onion juice Potato juice Sucrose solution Glucose solution Distilled water Reducing sugar solution Orange juice Egg albumen Honey Amino acid solution Protein solution Salad oil Vegetable oil Lipid solution RNA solution DNA solution Benedict’s solution Iodine solution Biuret reagent Sudan IV Acetone Brown wrapping paper Dische diphenylamine

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