Identifying Macromolecules Using Biochemical Tests

1891 Words Sep 13th, 2016 8 Pages
Identifying Macromolecules Using Biochemical Tests

Introduction
Lipids, nucleic acids, proteins, and carbohydrates are macromolecules, which also makes them organic compounds. Any macromolecule made of subunits in a living organism is an organic molecule. Carbohydrates are molecules of C, H, and O in 1:2:1 ratio, proteins are composed of amino acids, lipids are nonpolar molecules mostly C-H bonds and are usually hydrophobic, and nucleic acids are made of nucleic subunits, most identifiable as DNA/RNA. In today’s experiment we identify the presence of these macromolecules through a series of biochemical test and controlled experiments, which has a control used for comparison. Each of these molecules has a reagent that will bind with it and cause a reaction. Both the positive control, which contains the variable that is being tested for, and the negative control, or the solvent will be able to be identified.

Purpose
The purpose of this lab was to identify the presence of macromolecules in different solutions using different biochemical test and to understand what it means to have a control and the importance thereof.

Materials
Test tubes
Onion juice
Potato juice
Sucrose solution
Glucose solution
Distilled water
Reducing sugar solution
Orange juice
Egg albumen
Honey
Amino acid solution
Protein solution
Salad oil
Vegetable oil
Lipid solution
RNA solution
DNA solution
Benedict’s solution
Iodine solution
Biuret reagent
Sudan IV
Acetone
Brown wrapping paper
Dische diphenylamine…
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