Purpose: To use indicators to test for the presence of organic compounds in certain substances. Hypothesis: Honey will contain sugars, egg white contains proteins, corn oil contains lipids, oats contains starches and proteins, gelatin contains sugars and proteins, potatoes contain starch, and apple juice contains lipids and sugars. Materials: 9 test tubes Test tube rack Test tube holder Grease pencil Hot plate 20 ml honey solution 20 ml egg white and water mixture 20 ml corn oil Oats and water 20 ml gelatin and water solution 20 ml potato and water mixture 20 ml apple juice and water mixture 20 ml unknown substance #1 20 ml unknown substance #2 Paper towels 600 ml beaker Brown …show more content…
12. Record your observations then wash test tubes thoroughly. 13. To test for lipids, divide the piece of brown paper into 9 equal sections. 14. Write the name of one test substance in each section. 15. Rub a small amount of each substance onto its corresponding section. 16. Wait five minutes till dry. 17. Hold paper up to light. A translucent spot indicates the presence of lipids. Data: Substance Lipid Test Carbohydrate Test Protein Test Honey - Starch - Sugar + (orange Benedict) - Egg White - Starch - Sugar - + (violet Biuret) Corn Oil + Starch - Sugar - - Oats - Starch + Sugar - (black Iodine) - Gelatin - Starch - Sugar - + (violet Biuret) Potato - Starch + Sugar - (black Iodine) - Apple Juice - Starch - Sugar + (orange Benedict) - Unknown 1 - Starch - Sugar + (orange Benedict) - Unknown 2 - Starch - Sugar - + (violet Biuret) Analysis: In this experiment, every substance tested positive for at least 1 organic compound. Corn oil tested positive for lipids. Honey and oats tested positive for starch. Honey, apple juice, and unknown 1 tested positive for sugar. Egg white, gelatin, and unknown 2 all tested positive for proteins. So, not a single substance didn't test positive for at least 1 organic compound. All the indicators used in this experiment change color when in the presence of a compound; therefore, they chemically react with the compound. No errors were made during this experiment
Atoms are the basic units of matter and all life is based on them. Life on earth is based on the element carbon. It is a highly versatile atom able to form four covalent bonds with itself or other atoms such as hydrogen and water. Atoms combine to form molecules and those that are carbon based are referred to as organic molecules. Organic molecules occur in four different types in living cells; carbohydrates, lipids, proteins and nucleic acids. They are also known as hydrocarbons due to the presence of both hydrogen and carbon. Carbohydrates are made up of carbon, hydrogen and oxygen in the ratio 1:2:1. They are important sources of energy and are classified in three main groups; monosaccharides, disaccharides and polysaccharides.
The purpose of this lab was to test different substances using various procedures to see what biomolecules were present and ultimately find out what restaurant Anna Lyza had eaten at before she died. For the first control test, we used vegetable oil to test for lipids. So, if the solution does not contain lipids, it does not become translucent when placed onto a paper bag square and held up to a light. So, it is a negative result. However, in the presence of lipids, the solution will become translucent when placed onto a paper bag square and held up to a light. Therefore in this case, the result is positive. On the other hand, we used albumin egg to test for proteins in another control test. If the solution does not contain proteins, it will not experience any color change and so it is a negative result. When there are proteins existing in the solution, it will turn bluish/purplish and for this reason it is a positive result. Furthermore in the third control test, we used dextrose to test for simple carbohydrates such as glucose. If the solution does not contain simple carbohydrates, it will not undergo any color change and will remain a blue color. So, it is a negative result in this circumstance. If there are simple carbohydrates present in the solution, the solution will turn reddish and so the result is positive. For the last control test, we used starch solution to test
Purpose: The purpose of this experiment is to observe chemical changes in common consumer products to determine if the chemicals are basic, acidic, or remain neutral when mixed with other chemicals.
All living things contain some form of organic macromolecules including: Lipids, proteins, carbohydrates and nucleic acids. All of these organic molecules are alike in the sense that is they are made up of bonded elements such as carbon, hydrogen, oxygen, and to smaller quantities nitrogen, phosphorus and sulfur. The macromolecules each contain large long chains of carbon and hydrogen atoms and often consists of repeating smaller molecules bonded together in a repeating pattern (polymers). To test whether a specific solid white substance is a protein is simple due to the unique chains found in the respective type of macromolecule. To test if the substance is a carbohydrate, two tests could be performed on separate samples of the object. First,
The materials used for this experiment were, distilled water, albumin, pepsin, and starch. The liquids used to identify these organic molecules were Biuret reagent, Benedict’s reagent, and iodine solution. Biuret reagent is a blue colored liquid that identifies proteins or peptides in a solution. Benedict’s
The primary goal of this laboratory is to correctly identify an unknown substance. To achieve this task, one may use various tests that reveal both chemical and physical properties of a substance. By comparing the results of a known substance and the unknown substance, one may eliminate alternative possibilities and more accurately predict the undisclosed compound. Furthermore, by performing these tests, data can be collected and verified regarding chemical and physical properties of the unknown. Understanding the chemical properties of a known substance aids one’s understanding of the unknown based on comparative analysis of the results of the tests.
4. Describe the test used to detect the presence of each type of biologically important
2. Four unknown samples were included in the lab kit: flax seed meal, potato starch, egg whites, and dried milk. Using the results of the biochemical testing, determine which number corresponds to the correct unknown. (8 points)
7)a) You would do the Benedict’s reagent test for simple sugars and the Lugol’s solution or Iodine test for polysaccharides and starch. To test the lipids you would use the Sudan iV test and Biurets reagent test for protein.
Table 2: Consists of color extract taken from a red cabbage for a natural indicator. The pH reading that was measured by using the pH meter and the result of the pH reading to determine whether the solution was acidic or basic.
In another test tube, put 1 mL of hamburger meal slurry. Repeat steps 2 and 3 with the hamburger meal slurry and record the results in Table 2.
Identifying this organic acid was an extensive task that involved several different experiments. Firstly, the melting point had to be determined. Since melting point can be determined to an almost exact degree, finding a close melting point of the specific unknown can accurately point to the identification of the acid. In this case the best melting point
The guiding question of this ADI lab was, “What are the identities of the unknown compounds?” The goal of this lab was to understand the relationships between moles and molar mass to find the identity of unknown compounds. The mole can be used to measure small amounts of a substance or is used to convert from unit to unit using dimensional analysis. One mole is equivalent to the molar mass in grams of that substance. If you start with the moles of an unknown substance, multiply it by a given compound’s molar mass, and then divide it by however many moles are in the compound of your choice, you will get the mass of the compound. With that answer you can then compare with mass of the compound in the bag to determine its identity. We first started
Dispense .5 mL water into the already weighed conical vial, replace cap and face insert on its down side.
The physicochemical tests include 11 continuous variables, such as determination of alcohol, sulfates or pH values.