Identifying The Pathogenicity Or Possible Applications For Pharmaceutical Uses
964 WordsApr 18, 20164 Pages
Emily Corkum, TA
BIOL 3654 – L01
18 April 2016
Identification of Unknown 17
Three possible identities were provided for the unknown 17: Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli. Biochemical and molecular testing were used to identify unknown 17 as Pseudomonas aeruginosa. The ability to do this is extremely important because it provides knowledge about the structure and function of the microorganisms. The information can then be used to determine the pathogenicity or possible applications for pharmaceutical uses.
P. aeruginosa is a versatile gram-negative pathogenic bacterium, from the Proteobacteria phylum, that is capable of surviving unfavorable conditions. Although it is typically…show more content…
Utilizing the structure and metabolite information known about E. faecalis, P. aeruginosa, and E. coli, various biochemical tests were selected for the identification process. Six tests provided confirmation for P. aeruginosa and three tests provided confirmation for E. coli.
Gram staining differentiates between Gram positive and Gram negative bacteria and provides cell morphology. From this, pink bacilli were observed, providing a Gram negative result. Thus the unknown was narrowed down to E. coli and P. aeruginosa. Next was the Fluid Thioglycollate Media (FTM) which consists of a sugar broth and determines the oxygen requirements for the unknown. Since the turbidity was observed at the top of the tube, thus the unknown was an aerobe, just like P. aeruginosa and not E. coli which is a facultative anaerobe. Then a Durham tube with a glucose broth was used to determine whether the bacterium produces acid and gas from carbohydrate fermentation. Since only a red broth with some turbidity at the top was observed it could not have