Identifying The Unknown Bacteria From A Mixed Culture

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Identification of Enterobacter Aerogenes Annie Banh November 15, 2014 Jason Atkins Unknown #19 The purpose of this study is to identify an unknown bacterium from a mixed culture. We identified the unknown bacteria through different biochemical tests and identified the many metabolic capabilities of the bacteria. Studying and discovering unknown bacteria is very important in the health field as it may lead to new medicines and innovations for current, existing diseases as well as any upcoming diseases. The discovery of unknown bacteria is very useful in the pharmaceutical industry, as most of the products prepared are made based on these unknown organisms. The biochemical tests used to identify the unknown bacteria included: phenol red broth test (glucose, lactose, and sucrose), methyl red and Voges-Proskauer test, citrate test, indole test, H2S production test, urea test and motility test. The PR broths are used to differentiate members of Enterobacteriaceae and to distinguish these members from other gram-negative rods. The PR broths are useful in determining a bacterium’s fermentation abilities. If the bacterium is able to utilize the carbohydrate, then the acid production from fermentation lowers pH, which in turn turns the broth yellow. However, if the bacterium is unable to utilize the carbohydrate, but does utilize the peptone, which produces the by-product ammonia, the medium will have a rise in pH turning the broth pink. The methyl red and Voges-Proskauer tests
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