Igdi Case Study

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Abstract Nearly half of the world’s population is infected with Helicobacter pylori. Clinical manifestations of this infection range from gastritis and peptic ulcers to gastric adenocarcinoma and lymphoma. Due to the limited efficacy of anti-H. pylori antibiotic therapy in the clinical practice, there is increasing interest in the development of a protective vaccine against H. pylori infection. The bacterial protein FliD forms a capping structure on the end of each flagellum which is critical to prevent depolymerization and structural degradation. In this study, the potential of FliD as a prospective H. pylori subunit vaccine was assessed. For this purpose, immunogenicity and protective efficacy of recombinant FliD (rFliD) from H. pylori…show more content…
Antibiotic therapy, although effective, is associated with several disadvantages including the low patient compliance due to the drugs’ side effects, treatment failure as a result of the emergence of drug-resistant strains, high costs, and the failure to prevent reinfection [4-7]. Therefore, alternative approaches to combat H. pylori infection are currently pursued, including vaccination. Several H. pylori proteins have been identified as immunogenic in preclinical models, including Urease B (UreB) [8], Vacuolating toxin A (VacA) [9], H. pylori adhesion A (HpaA) [10], neutrophil-activating protein A (NapA) [11], outer membrane protein (Omp) [12], cytotoxin-associated antigen (CagA) [9], heat-shock proteins (Hsp) [13, 14], OipA [15] catalase [16] and chimeric genes [17-19]. Each of these antigens has the ability to reduce the bacterial load in animal models, but none affords protection against infection. Current strategies to enhance vaccine efficacy include the identification of most suitable immune targets and the combination of immunodominant antigens into multivalent formulations. Gholi et al. [20] demonstrated that FliD,, the flagellar hook-associated protein 2, reacts with approximately 97 percent of sera obtained from patients infected with H. pylori, suggesting that this is a common immune target in the infected human host [20]. FliD plays a crucial role in flagella assembly. Flagellin is important for bacterial motility and is essential for
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