Immunohistochemistry: Antigen Detection in Tonsillitis Essay

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Antigen Detection in Tonsillitis Using Immunohistochemistry
Tonsillitis can be described as the inflammation of the non-encapsulated lymphoid structures, lingual and palatine tonsils. These lymphoid tissues are part of the immune system and are the first line of defence against pathogens in the oral cavity. The palatine and lingual tonsils are located underneath the stratified squamous epithelial mucosa of the tongue and oropharynx. The tonsils’ response to bacterial or viral infections of the epithelial mucosa, e.g. streptococci and the Epstein-Barr virus (EBV), is inflammation and enlargement of the tonsils and antibody responses, largely IgA. Symptoms of tonsillitis include Sore throat and fever.
Immunohistochemistry (IHC) is a method
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Antigen Detection in Tonsillitis Using Immunohistochemistry
Tonsillitis can be described as the inflammation of the non-encapsulated lymphoid structures, lingual and palatine tonsils. These lymphoid tissues are part of the immune system and are the first line of defence against pathogens in the oral cavity. The palatine and lingual tonsils are located underneath the stratified squamous epithelial mucosa of the tongue and oropharynx. The tonsils’ response to bacterial or viral infections of the epithelial mucosa, e.g. streptococci and the Epstein-Barr virus (EBV), is inflammation and enlargement of the tonsils and antibody responses, largely IgA. Symptoms of tonsillitis include Sore throat and fever.
Immunohistochemistry (IHC) is a method of finding antigens in tissue cells by taking advantage of the principle of specific antibody –antigen binding. IHC is an important diagnostic tool, diagnosing atypical cells, e.g. cancerous cells in tumours. Specific molecular markers are used to distinguish cellular actions, e.g. proliferation and apoptosis. Extensively used by researchers to figure out the distribution and localization of biomarkers and proteins in organic tissue. IHC involved the conjugation of an antibody with an enzyme, e.g. peroxidase, able to catalyse the reaction that produces colour.
The IHC procedure included paraffin sections that were de-waxed and pre-treated before staining. Hydrogen peroxidase was used to block endogenous peroxidase activity. Non- specific
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