Immunohistochemistry: Antigen Detection in Tonsillitis Essay

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Antigen Detection in Tonsillitis Using Immunohistochemistry Tonsillitis can be described as the inflammation of the non-encapsulated lymphoid structures, lingual and palatine tonsils. These lymphoid tissues are part of the immune system and are the first line of defence against pathogens in the oral cavity. The palatine and lingual tonsils are located underneath the stratified squamous epithelial mucosa of the tongue and oropharynx. The tonsils’ response to bacterial or viral infections of the epithelial mucosa, e.g. streptococci and the Epstein-Barr virus (EBV), is inflammation and enlargement of the tonsils and antibody responses, largely IgA. Symptoms of tonsillitis include Sore throat and fever. Immunohistochemistry (IHC) is a method…show more content…
Monoclonal mouse anti-human CD68 labels human monocytes and macrophages. CD68 molecule was present on patient intrafollicular tonsil macrophages, distinguished by the brown colour. Monoclonal Mouse anti-human CD20 reacts with an antigen which is a very useful marker for normal and neoplastic B cells. The B-lymphocyte antigen CD20 causes B-cells to stain brown in the IHC practice, intrafollicular B-cell presence is most notable, however cells stained brown are also present in the interfollicular zone. CD3 expressed in peripheral T cells, region of brown in dark purple cells lining follicle, very faint. diH20 acted as a negative control in the experiment, all cells, both in intrafollicular zone and in interfollicular zone stained purple. Cells stained brown in the negative control indicate non-specific staining. KI-67 shows B-cell proliferation presented as pink cells, largely in the intrafollicular zone but also in the interfollicular zone, indicative of a humoral adaptive immune response. Tissue sample presented with large population of B cells, labelled with CD20, in follicles and macrophages labelled with CD68 were also visible in the intrafollicular zone, both are indicative of a humoral immune response. 1. Ramos Vara JA, Miller MA. When Tissue antigens and antibodies get along: revisiting the technical aspects of immunohistochemsistry- the red, brown and blue technique. Vet Path. 2014; 51(1):42-87. 2. Price P.

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