Impact Of Toxicity Of Commercial Sodium Salt On Kidney And Dna Of Male Mice Part 1
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Impact toxicity of commercial Sodium Chlorid Salt on Kidney and DNA of male mice Part 1
1*Aly, H. M. 2Gamela S.M. El-Saeed, 3Rizka K.Ali.
1Department of Cell Biology,
2Department of Medical Biochemistry,
3Department of Water Pollution Research,
Abstract: Sodium chloride is widely used in various industrial, medical and food applications that increasing human exposure to it by excessive amount. However, almost all studies focused on its nephrotoxicity and adaptation of kidney cells to high concentration salt and only few scientific attentions were concerned on its cytoxicity. Hence, the oxidative DNA damage induction by excessive salt intake in mice was investigated. mice were orally administrated with three different concentrations commercial NaCl solution started with concentration at tap water. Second and third concentrations were (50 & 100mg/L) orally administrated for 10 successive days then sacrificed 24 hour after the last orally administrated. Comet assays were used to evaluate the possible toxicity of excessive salt on DNA levels. Also, some biochemical markers of kidney were assessed to shed more light on salt mechanism of action. DNA damage induction by excessive intake of high salt was evidenced by the statistical significant elevations in both two group. This was further evidenced by appearance damage of DNA on agarose gel. Moreover, excessive NaCl caused significant elevation in kidney function level in a dose dependent manner. It was also noticed that