Induced Pluripotent Stem Cells Essays

These cells come from fetuses which is why stem cells are such a controversial issue. In order to actually use embryonic cells, they must be harvested from an unborn baby that is in the early stages of life. Embryonic cells have the ability to be all 220 types of tissues in the human body. “This makes them ideal for regenerating diseased heart tissue, repairing spinal cords, and replenishing brain cells”, Elizabeth Svoboda, an author in the publicized Popular Science magazine, illustrates in her article about stem cells (60). Embryonic cells have limitless possibilities in medical advancement. These cells can become whatever type of cell a doctor program them to be. With the use of embryonic stem cells, doctors can cure several diseases, as well as curing people who have suffered injuries, but research is being slowed down by the ethical dilemma of aborting a

Pluripotent stem cells are the stem cells that can only differentiate into a limited range of differentiated cells. (2) They have the ability to give rise to all somatic cells from ectoderm, mesoderm and endoderm, as well as gametes. Naturally it can be found in embryos as Embryonic stem cells (ES cells). Induced pluripotent stem cells (iPS cells or iPSC) are the pluripotent stem cells that are generated directly from adult cells, first discovered by Shinya Yamanaka in 2006 by using a set of reprogramming factors (Oct4, Sox2, Klf4, and c-Myc or LIN28 and Nanog) (3) to reprogram mature cells back to a pluripotent state (4).

Embryonic stem cells (hESC) are pluripotent. They are obtained from the inner mass of a 5-6 day old human blastocyst that consists of approximately 100 cells (Bongso & Lee, 2005, p. 3).

Embryonic stem cells are found in human blastocysts (Marcovitz 17). A blastocyst is a very young embryo (just a few days old) that contains around 200 undifferentiated stem cells (Marcovitz 17). German Zoologist Valentin Hacker coined the term “stem cell” after he discovered them in a blastocyst of a crustacean (Marcovitz 18). Embryonic stem cells were collected for the first time in 1988 by Dr. James Thomson of University of Wisconsin and by Dr. John Gearheart of Johns Hopkins (Panno 76). These stem cells are unspecialized; they do not perform a specific function like cells such as muscle and nerve do (“Stem Cells”). They are also pluripotent, meaning they have the ability to divide and become specialized cells (“Stem Cells”). This is why stem cells hold so

Recently in the scientific world, the field of embryonic stem cell research has become a popular topic and has been the subject for many heated debates. Experts in the field of stem cell research promise that this will be the future of medicine; that stem cells will be the cure to all the debilitating diseases and afflictions of today, such as Alzheimer’s disease, heart disease, cancer and nerve damage. The truth about embryonic stem cell research is that it is not as hopeful and as revolutionary as it seems. Many problems that will negate the use of stem cells will and have already begun to arise, especially in areas concerning health risks to the patient, opportunity costs and human rights (Condic 803). Taking into account all the

Embryonic stem cells (ESCs) are grown in the laboratory from cells found in the early embryo. ESCs have an unlimited chance to

The 70's focused on research that involved fetuses in utero. The 80's shifted to research of transplantation of fetal tissue into adults with serious medical conditions such as diabetes, Parkinson's, and spinal cord injuries. The latest saga involves using cells from days-old "spare" embryos that are created in infertility treatment process and all are considered unethical and unmoral issues.

Keywords: ethical, embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), disease, drug development, research

All the human embryonic stem cell lines currently in use come from four to five-day-old embryos left over from in-vitro fertilization (IVF) procedures. In IVF, researchers mix a man 's sperm and a woman 's eggs together in a lab dish. Some of those eggs will become fertilized. At about five days the egg has divided to become a hollow ball of roughly 100 cells called a blastocyst which is smaller than the size of the dot over an “i”. ("Myths and Misconceptions About Stem Cell Research.")

Human embryonic stem cells (hESCs) are pluripotent and are obtained from the inner mass of a 4-5 day old human blastocyst that consists of approximately 100 cells (“Stem cell research,” 2009).

The creation of induced pluripotent stem cells by direct reprogramming has allowed for the circumvention of using embryonic stem cells while still leaving the cells with the ability to maintain pluripotency. Instead of ES cells which were originally derived from the epiblast of mouse embryos, IPS cells were generated from mouse embryonic fibroblasts. This eliminated both any ethical concerns for whether those cells were a living being or not and the need to destroy embryos at the blastocyst stage. An advantage of IPS cells is that they are derived from human somatic cells which makes them easy to acquire due to the possibility of using skin or blood cells. They can also be grown and differentiated individually for each person that the sample of somatic cells is taken from which eliminates the possibility of having any immune reaction and rejection to the differentiated cells during transplantation. These characteristics of IPS cells are important because they are what enables us to safely and accurately transform these affected cells from patients cells into neurons and confidently study them.

Induced pluripotent cells from somatic cells by transduction, DNA-based induction, RNA molecules and recombinant proteins, creates a reprogramming wave leading to cells with the potential to become many cell types (16). The equivalency of induced pluripotent stem cells and embryonic stem cells is variable but using screening techniques, bioinformatics assay, cells that are more similar to embryonic stem cells can be selected for cloning techniques (18,19). The reprogramming wave, occurs in two parts, driven by c-Myc/Klf4 (first wave) and Oct4/Sox2/Klf4 (second wave),

There are several new methods that have been developed since the start of the highly controversial stem cell debate which rectifies the major differences on both sides. New solutions such as Induced Pluripotent Stem Cells (iPS) acts as an alternate method to embryonic research in that it uses cellular reprogramming of adult skin cells.“The benefit of iPS is that stem cells can be created without the use of embryos, however, the cells resemble embryos in that they can, theoretically and under the appropriate conditions, be made to differentiate into any type of cell found in the body ” (Phillips, 2010). . There are also techniques being developed that use amnionic fluid, or stem cell extraction techniques that do not damage the embryo, that also provide alternatives for obtaining viable stem cell lines ” (Phillips, 2010). The only caveat to all of these newly developed alternatives is that no solution has been studied long enough to claim that it can be an effective substitute 100%. “To begin with, demand for

As technology advances, the use of embryonic stem cell research has also expanded. Stem cells have shown promise in personalized medicine as they are undifferentiated and easily conform with the surrounding cells. There are two areas of research that stem cells are showing massive potential, cell regeneration and organ transplantation. It is thought that stem cells have the capability to “model genetic disorders in a reliable fashion such that no other method allows. It seems likely that we could use stem cells to model cells with genetic disorders and figure out how to mute certain genes, thus eliminating or drastically reducing the effects of the disease,” (1). Although embryonic stem cells (ESC) are showing great potential towards medical advancements, there are many people who are opposed to the idea of using these cells due to the aggressive nature in which we extract ESC.

Embryonic stem cells (ESCs) are derived from a week old human embryo (blastocyst), often developed from unused in vitro fertilised eggs. They are pluripotent, meaning they are capable of undergoing directed differentiation and developing into a plethora of cell types. They have to potential therefore to cure a vast number of diseases, including Parkinson’s, diabetes, spinal cord injury and heart diseases (V. Rebecca, 2013). Those who support the ECS research state that the week old blastocysts are purely a cluster of cells, not a human being. However those who detest the research view the week old blastocysts as an embryo, a human life. This is where the parties differ in arguments and ethical values, therefore bringing about the heated debates.