Interaction Between Y-Cyclodextrin And Pyrene

The unknown sample was identified to contain Acetaminophen, Aspirin, and caffeine based on comparison of known Rf values (Table 1). When comparing the traveled UV lit spots (figure 1), Aspirin and Ibuprofen traveled the same distance from the start line. Unknown sample 16 contains Aspirin not Ibuprofen, due to the intensity of the spot under UV light. Aspirin and the unknown shared that same intensity, whereas Ibuprofen did not share that same strength of brightness. The distance traveled by the samples was dependent upon their affinity for the mobile phase 200:1 ethyl acetate – acetic acid. The samples that traveled the furthest like the Aspirin and Ibuprofen were the most soluble in the mobile phase. The samples that traveled the shortest

This experiment was used to measure the buildup of the colored product, benzoquinone, to observe the change in the absorbance of the mixture in a spectrophotometer at a wavelength of 486 nm. It was hypothesized that over a longer period catechol oxidase activity and reaction in the mixture will continue, creating more benzoquinone resulting in an increase in the absorbance. The hypothesis was supported by the data as seen in figure 2 which shows the positive relationship between time and absorbance. As can be seen, with more time the catechol oxidase can catalyze the reaction and turn catechol (substrate) into benzoquinone (product). This in turn increased absorbance of the blue light at 486 nm by the solution containing benzoquinone which has a dark brown

Different reagents were used for the tests with the reagents reacting with the molecules in a specific way indicated by a change of color. Controls were provided to ensure integrity of the process. The results indicated the presence or absence of the organic molecules in the various substances provided. They proved that using these tests, one can accurately determine what organic molecules a substance contains. This will help in determining the composition of various substances within the cell or even outside the body and how they affect normal cell

After placing in darkness the colorless solution resulted by reformation of the radical intermediates to a new thermodynamic product via C-N bond at room temperature. UV-Vis was conducted on the solution before and after the irradiation with sunlight: UVtoluene 554.92nm, A=0.12 before irradiation and A=1.05 after. The peak at 554.92nm corresponds to yellow/green light and its complementary colour is red/violet. This validates the solutions violet color. The increased absorbance was accounted for an increase in the radical component. The radical was formed when exposed to light, which was visually apparent with the purple coloration and proved the thermodynamic dimer was also photochromic. When dimer 4 is exposed to light photons collide with the molecule and impart energy upon them. This energy is significant enough to break the bond between the two rings and results

Evers, D. J., B. Hendricks, G. Lucassen, and T. Ruers. "Optical Spectroscopy: Current Advances and Future Applications in Cancer Diagnostics and Therapy." NCBI. National Center for Biotechnology Information, 8 Mar. 2012. Web. 13 Sept. 2015.

Both Aspirin and the Unknown are soluble in dichloromethane, due to their non-polar characteristics. To separate the two components, sodium bicarbonate was added (see figure 3). Sodium bicarbonate reacted with aspirin and converted it to a salt, also forming water and carbon dioxide. It was observed that the solution "fizzed" when this reaction took place, showing the release of carbon dioxide. The newly formed salt then traveled to an aqueous layer where it was soluble, while the unknown remained in the dichloromethane layer. The two layers were then separated. To collect an aspirin solid, the combination of the addition of HCl and the process of vacuum filtration helped to break down the salt and form a solid. Then the solid was placed in the Fisher Scientific Biotemp Oven to dry it to a constant mass of 0.091 g, 32.97% of the total composition. The

Therefore wide range of analytical methods for quantitation of DXM have been developed such as, high performance liquid Chromatography(HPLC) (Abdel-Moety, Al-Deeb, and Khattab 1995, Tedesco et al. 2013, Eichhold et al. 1997, Hoskins, Shenfield, and Gross 1997) (Abdel-Moety, Al-Deeb, and Khattab 1995, Tedesco et al. 2013, Eichhold et al. 1997, Hoskins, Shenfield, and Gross 1997) 7), thin-layer Chromatography(Singh and Moody 1995)(Singh and Moody 1995)

The goal of this experiment is to prepare a photosensitive solution and explore its properties. While analyzing the solution, one will learn how to successfully handle these sensitive chemicals and then establish its properties via spectrophotometry.

1. Purpose: to clarify the mechanism for the cycloaddition reaction between benzonitrile oxide and an alkene, and to test the regiochemistry of the reaction between benzonitrile oxide and styrene.

In the Dyes and Crimes laboratory experiment, the phosphorescence, fluorescence, and chemiluminescence properties i.e. traits of several chemicals were examined using (UV) Ultraviolet lamp. Observations on color, intensity, duration of glow, etc. were analyzed to determine the traits of the several chemicals. Correspondingly, the author of the unsigned note was determined through ink extraction, (TLC) Thin Layer Chromatography, (UV-Vis) Ultraviolet-visible spectroscopy along with the Rf values for of each individual sample of ink compared to the unknown: the ink of the author on the unsigned note. Phosphorescence substances

AAS has contributed to the understanding of elements having different absorption emission spectra due to their difference in energy levels. In the absorption spectrum, the absorbed light are shown as black gaps. As the number of electrons increase, the number of spectral lines also increase. Hence, by measuring the absorption of light, the concentration of the element within a sample can be determined. By knowing the concentrations of an element, scientists are now aware that even the smallest amount can make a significant impact towards the biological system. Therefore, scientists have brainstormed ways to monitor the use of chemicals in the

On a thin chromatography plate, five spots were placed ( as shown in table 2) and the plate was developed using chloroform/methanol. This was later visualized with dragendorff’s reagent under the UV light. All separated components were observed, identified and recorded.

The light yellow precipitate was collected by suction filtration using a Hirsch funnel. The product was washed with two 1-mL portions of cold methanol followed by two 1-mL portions of diethyl ether. The product was dried in the oven at 110°C. The IR spectrum as a KBr pellet was obtained for the product and inosine for analysis.

In this experiment, the use of a glass cuvette was used in order to avoid any chemical or environmental interference from the surroundings. Furthermore such glass cuvettes can support large wavelengths during transmission of light beyond 320 nm since this is the excitation wavelength region. Riboflavin is excited at around 370 nm, therefore the wavelength range must be large in order to detect a proper response. Plastic cuvettes are however inadequate as the range of wavelengths supported for transmission of light is very limited (5, Upstone). The geometry of the instrument of the spectrophotofluorometer has a specific angle and position for the excitation source and the detector. The excitation polychromator emits ultra-violet radiation and light to the interested sample and the shape of the polychromator resembles a triangular prism in which the monochromator has been angled at a 90∘ position. The transducer would detect the irradiation excitation beam and transfers it through the emission polychromator. The emitted beam would then travel to the detector to allow for the response signal to appear for the sample on the computer system (416, Skoog). The position of the cuvette is essential as it enables for the consistent readings of the response signals, as any shift in the cuvette position can create variation in results due to the position of lighting and detection of molecular samples hitting at different angles. The process of fluorescence is quite sensitive than absorption due to having lower detection limits because of the fact that it can detect lone molecules in excited states that are irradiated by UV-light unless they come into contact with other molecules within

By Using specific methods of compounds detection, we can match an unknown compound with a known compound because similar compounds will display similar characteristics. In this experiment, identifications of the unknown ketone was accomplished using thin layer chromatography, melting point, and NMR spectrometry. Thin layer chromatography is very quick but sensitive way of determining the components of a mixture or a specific compound from a list of knowns. This method is the one which was utilized in the