Introduction Of Klebsiella Pneumoniae Is A Gram Negative Bacillus Shaped Bacterium

The Gram stain was used to determine if the bacteria was gram positive or negative. A negative test shows a pink color and a positive test is a purple color. When a bacterium is negative it is because it has an outer membrane and a thin layer of peptidoglycan that is much harder to stain. A positive bacterium has a thick layer of peptidoglycan and no outer membrane that can be penetrated by crystal violet.

As a facultative anaerobe, Klebsiella is usually found in human intestines or stool, where it is harmless. However, it has recently become a more important pathogen if it gets in the respiratory tract or bloodstream of a person with poor health or a compromised immune system (Guentzel, ). It commonly causes nosocomial, or hospital-acquired infections, which are contracted through the contaminated hands of healthcare workers, catheters or other equipment that is not sterile. These infections normally occur in patients that are being treated in the hospital for some other reason. Some examples of Klebsiella induced syndromes are Urinary Tract Infections, bacteremia, pneumonia, diarrhea, meningitis and osteomyelitis. According to the Pennsylvania Department of Health (2017), the mortality rate of a Klebsiella infection is around ninety percent in most countries. Additionally, it has become increasingly hard to treat infections caused by Klebsiella due to its ability to resist certain

The two bacteria from sample number 118 I have identified to be Streptococcus mitis and Klebsiella pneumoniae. When taking a class like Microbiology it is understood that most individuals will progress further into the medical field. In the medical field it is important to know as much about as many different bacteria as possible.

This experiment was given to us to utilized previous knowledge learned throughout the semester to identify a gram negative unknown bacterium. We had to first learn the difference between a gram negative and a gram positive organism. We started off with doing gram stains to determine whether it was positive or negative. Based on the gram stains, a gram positive stains purple and a gram negative stains pink. A gram positive stains purple because the cell walls is made of a thick peptidoglycan layer and doesn’t

Bacillus cereus is a Gram-positive, facultative aerobic, spore-forming, rod-shaped bacterium that is widely distributed among the environment.

The pathogen in the NPR story was called Klebsiella pneumoniae, which is a Gram-negative coccobacilli, relatively small, (0.5-0.8/ 1-2um), does not form spores, and is easily fixed. ("Klebsiella Pneumoniae Morphology") This pathogen is arranged either singly or in pairs and clusters, and can be cultivated on ordinary media. On agar media, the bacteria forms grayish-white colonies which

The gram stain test was able to identify the bacterium as a gram-positive organism, and the morphology distinguished it as a strain of streptococcus, and these finds helped direct the other tests. Hemolytic tests are very useful in differentiating specific species of a certain family of bacteria. The hemolytic test showed the bacterium to be of alpha clearing, which meant it only partially lysed the red blood cells and left a greenish tint. This identified the bacterium as either strep pneumoniae or faecalis. The bacitracin test, which tests whether the bacterium is susceptible to the antibiotics in the disk, posted a positive

Morganella morganii is a gram-negative bacillus with no special arrangement. It is the third member of the tribe Proteeae. This bacterium was first discovered in the year 1906 by a British bacteriologist by the name of He. De R. Morgan. In the late year of 1939, the bacterium was named Proteus morganii, and again changed some years later due to findings that this bacterium did not obtain the ability to ferment all carbohydrates like the genus Proteus was capable of doing. Instead, researchers found the bacterium to have the capabilities of ferment only glucose and therefore its name had been changed one final time to Morganella (its own genus) morganii. While testing M. morganii, findings show that it has its own special characteristics that differ from the usual Proteea. M. morganii does not swarm on a nutrient agar plate like the typical Proteus would. It also does not produce the black precipitate found in Hydrogen Sulfide gas tests. M. morganii produces phenylalanine deaminase, which is the enzyme that wipes out the amino group, resulting in a phenyl pyruvic acid. It is a facultative anaerobe meaning that it is capable of producing energy in the form of ATP by aerobic respiration if oxygen is present in its environment. If oxygen is not present in the environment, the bacteria is fully capable of producing energy in anaerobic environments as well. Morganella morganii can be found in the soil, water, and feces. The bacteria is a common resident to the

In the world of microbiology it is vitally important to be able to discern the identities of microorganisms. Not only is it important in a lab setting but as well as in healthcare in general. Properly identify what strain of bacteria a person has will aid in the proper medicine and dose given. Throughout the semester we have learned about different types of bacteria and certain test that can clearly identify them. The purpose of this lab report is to identify a Gram-positive or Gram-negative bacterium. Using all the knowledge of procedures and lab techniques identify the unknown and discuss all the tests you performed.

Escherichia coli also for short E.coli, is a rod-shaped, gram-negative bacteria, this bacteria is usually motile and grows best at 37℃, because this is the temperature of the human body where it is normally found, because it is a bacteria that can be found in our normal flora it is a bacteria that is needed in our

The bases of this experiment was to discover the identify of the unknown from three possible specimens: Klebsiella pneumonia, Escherichia coli, and Enterobacter aerogenes. Utilizaing the T streak technique, the bacteria was isolated into pure colonies for further study. The Gram Stain method was used to identity the morhphology of the bacteria such as the shape and whether the bacteria was Gram positive or Gram negative. Biochemical test were also used to help identify the unknown bacteria. The biochemical test used was the Triple Sugar Iron Agar, Sulfur Indole Motility test, Methyl Red test, Voges-Proskauer test, Citrate test, Urease test, and the Gelatin test. After observing the morphology of the bacteria using the Gram Stain method and utilizing all the possible biochemical test, the bacteria was identified to be Enterobacter aerogenes.

pneumoniae) leaving me with Sa, Se, Ef, P.pyo, and Ml. Following my dichotomous key, the next test I decided to perform was a catalase test in order to determine if the enzyme catalase is produced by the bacteria. The sample of the bacteria I took did bubble, this indicates that the bacteria does produce the catalase enzyme as well as requiring oxygen to respire. Based on the results of the catalase test there were three possible microbes I could have Ml, Sa, and Se. For my next test I decided to use an MSA plate to determine if my unknown microbe could or could not grow in the presence of the mannitol salt, along with testing to see if it would or would not be able to ferment the mannitol salt. After allowing the bacteria to grow, I noted that the plate remained the same pinkish color it originally was however, I did several white colonies growing on the plate. The pink color that remained shows that the organism is unable to ferment the mannitol salt thus the acidic byproduct that turns the medium yellow will not be produced. Therefore the organism cannot be Sa since that

Klebsiella pneumoniae is a gram-negative bacilli bacterium 0.3-1.0um in diameter and 0.6-6.0 um in length. Cells are capsulated and arranged in pairs or in short chains. K. pneumoniae can cause different types of healthcare associated infections that include pneumonia, blood, and wound infections along with meningitis. K. pneumoniae is normally found in the intestines of human stool. Infections are most common with people who require ventilators, intravenous catheters or patients taking long courses of antibiotics. The primary portal of entry is through the respiratory tract to cause pneumonia or through the blood to cause bacteremia. People with healthy immune systems usually do not get k. pneumoniae infections but should

-B2 was Gram positive cocci that seemed to cluster and also showed no growth on the EMB plates.

Streptococcus pneumoniae is found worldwide. The common host is the human body, in which it often does not cause disease but at other times it can cause diseses in particular, pneumonia. It also causes otitis media, bacteremia, meningitis, peritonitis, and sinusitis. The route by which this organism is spread is from human to human in the form of aerosol droplets. When inside the host the organism’s primary site of pneumococcal colonization is the nasopharynx. From this site it can aspire to the lungs, eventually spread to the blood and traverse the blood-brain barrier to the meninges, once inside the blood it can cause infections throughout the body. Symptoms of the disease include sudden