Introduction to Prokaryotes Lab Report

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Introduction to Prokaryotes in Different Temperature Environments

Angelina Orozco
Biology 1108
Wednesday 7:30 am
Prokaryotes are single-celled organisms that can survive in extreme environments. Bacteria is the more numerous type of prokaryotes. The group hypothesizes that the samples taken from different environments will all cultivate diverse morphology in fast growing rates in each environment. The aseptic technique was used to cultivate bacteria from different environments. The diversity of morphology and the growing rate of the bacteria was different in each environment.
Prokaryotes are the oldest known life-forms, having existed for the last 3.5 billion years. Microscopic in size, they are single-celled
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Every environment sample had its own growth rate and bacteria diversity. The primary reason may be that conditions are rarely optimum. Scientists who study bacteria try to create the optimum environment in the lab: culture medium with the necessary energy source, nutrients, pH, and temperature, in which bacteria grow predictably. Most of the strains used in the classroom either require oxygen for growth or grow better with oxygen. These bacteria will grow better on agar plates, where air readily diffuses into the bacterial colony, or in liquid cultures that are shaken. Since diffusion of oxygen into liquid depends on the surface area, it is important to have a large surface; volume ratio. This means that cultures will grow best in flasks in which the volume of liquid is small relative to the size of the vessel. Also another factor that affects growth is the nutritional medium. Bacteria grow best when optimal amounts of nutrients are provided.
Tables and Figures

Plate | Total # Colonies | Colony Morphology | Color | 1 | 7 | 6-circular/flat/entire
1-circular/convex/entire | 1-yellow
4-white | 2 | 1 | 1-circular/convex/entire | 1-yellow | 3 | 77 | 5-irregular/undulate/lobate
20-irregular/flat/undulate | 13-white
2-orange | 4 | 57 |
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