Investigate the Factors That Affect the Rate of Respiration in Yeast

1941 Words Aug 22nd, 2010 8 Pages
Mashrek International School

Investigate the Factors that Affect the Rate of Respiration in Yeast. (Temperature)

Fawzi El Ansari

Biology HL

Title: Investigate the Factors that Affect the Rate of Respiration in Yeast. (Temperature)

Aim: The aim of this experiment is to investigate the effect of changing the temperature on the rate of respiration in yeast. This will be done by placing equal amounts of yeast in each beaker that contains the same pH solution. Each beaker will be mixed with glucose solution and then will be placed at a different temperature in which the amount of CO2 produced will be measured every one minute.

Rationale: As we all know, respiration is a process that takes place in all living organisms where it converts
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| Volume of CO2 produced from yeast during respiration at temperatures/mL (+/-0.5) | Time/min.(+/- 1) | 35/C | 40/C | 50/C | 60/C | 0 | 0.0 | 0.0 | 0.0 | 0.0 | 1 | 0.0 | 8.0 | 28.0 | 10.0 | 2 | 0.0 | 16.0 | 40.0 | 14.0 | 3 | 0.0 | 34.0 | 54.0 | 14.0 | 4 | 0.0 | 50.0 | 70.0 | 14.0 | 5 | 0.0 | 64.0 | 80.0 | 14.0 | 6 | 10.0 | 80.0 | 92.0 | 14.0 | 7 | 10.0 | 98.0 | 106.0 | 14.0 | 8 | 10.0 | 110.0 | 108.0 | 14.0 | 9 | 18.0 | 130.0 | 108.0 | 14.0 | 10 | 20.0 | 144.0 | 110.0 | 14.0 |

Observations: During the experiment, bubbles were present from the water in the electric water baths that were set at temperatures of 50 degrees Celsius and 60 degrees Celsius. This is because the water has passed its boiling point and began to boil. The glucose powder had a white color. The yeast powder had a mild yellow color. When the glucose solution was mixed with water, it gave a clear color. When glucose solution was mixed with the yeast, it produced a murky yellow color. Bubbles also arose when CO2 was being lost in the experiment under each desired temperature.

Graph 1: Volume of CO2 produced from different temperatures and the time recorded each minute.

Data Processing:
Table 2: Slope of different Temperatures of the measured results of the volume of CO2 produced from yeast during respiration. Different Temperatures/C(+/- 1) | Rate/cc/min.(+/-0.01) | 35 | 2.11 | 40 | 14.98 | 50
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