Investigating If Liver And Potato Cells Contain Natural Buffers That Resist Large Change
772 Words4 Pages
Purpose: The procedure of this lab is to determine if liver and potato cells contain natural buffers that resist large change in pH as 1. NaCl or 1. NaOH are added to the solution.
Hypothesis: If adding an acid or a base to liver and Potato cells then the solutions will become buffers.
Materials: pH paper- cut into chunks
0.1 Molar NaCl
0.1 Molar NaOH
Commercial Buffer Solution- pH 7
Liver homogenate- 10g per 100mL of water
Potato homogenate-10g per 100mL of water
25 mL graduated cylinder
Put on safety goggles.
Wash beaker to clean out any left behind substances.
Pour 25 mL of distilled water into the 50 mL beaker.
Cut pH slips into thirds.
Dip pH slips in the solution.
Once out, record its initial pH by comparing it to the key.
Add one drop of HCl into the distilled water.
After added, pick up the beaker and swirl it around lightly for a short period of time.
Take the pH level again.
Continue this until 10 drops have been added ( one at a time).
After that, continue to find the pH levels, but for every five drops.
Keep adding five drops of HCl or the solution until a total of thirty drops have been added. Once done, redo this procedure, but uses NaOh to drop into the solution.
After complete, continue using HCl and NaOH, but now testing potato cell, liver cell, and the buffer (one at a time)
Analysis: The Distilled water pH average of HCl for zero drops was 6.67, and the pH for the final thirtieth drop