Investigating If Liver And Potato Cells Contain Natural Buffers That Resist Large Change

In this lab, the purpose was to determine the stability of a substance after adding an acid or a base. The results claim that liver and buffer are the most resistance to change in pH. Looking at figure 3, buffer and liver both maintain a stable pH even with the addition of an acid or base. However, potato and water have less buffer in them since their pHs did change. In figure 3, the potato acid’s pH level decreased by two, and the potato base’s pH level increased by two. The level of pH of a water acid decreased by 4, while the water base’s pH increased by 5. These results all tie to the fact that buffer is a substance that maintains a stable pH; the presence of buffer in organisms help maintain homeostasis by binding or releasing hydrogen

You may have observed the reaction of naturally-occurring catalase in tissue from either liver of potato. Design an experiment to determine if the amount of catalase varies from tissue to tissue (e.g. 200 g of liver compared to 200 g of potato).

H. How would you prepare 10 mL of a 0.25M HCl solution if 1M HCl was available? How much

Purpose: To determine the concentration of solute in the potato’s cytoplasm by measuring the change

Once this has happened, you will remove the solution from the saucepan, pour the solution into a container and label that container 20% sugar solution.

lab bench. Place a beaker from the drawer on the stir plate. Drag the bottle of NH3 to the 5 mL graduated cylinder (the smallest one) by the sink and fill the cylinder by dropping the bottle on the cylinder. Now drag the 5 mL graduated cylinder to the beaker on the stir plate and add the 5 mL of

To prevent fluctuation in the pH, a solution known as a “buffer solution” was used in the experiment. Buffer solutions are mixtures of at least two chemicals which counteract the effect of acids and alkalis. Therefore, when a small quantity of alkali or acid solution is added the pH of the enzyme doesn’t change.

The homogenate used in this experiment was liver homogenate. Five different samples and a blank

ii. The second part of the titration series involves titration of NaOH with Hydrochloric acid (HCL). Again, three reps of titration and a blank titration have to be completed. A volumetric pipet is used to measure 10.00mL of HCL into three labeled conical flasks. Then the flasks are filled with deionized water until about the 50mL mark. A buret is

1) Separate the solid from the liquid in the beaker by decanting the liquid. Ask your instructor to demonstrate the correct procedure.

A total of 1.5 mL was added to produce a pH of around 6. The mixture was stirred for an additional 30 minutes.

The experiments involved PH buffers of different pH were added to potato juice, water, and the enzyme catecholase. The mixture was then subjected to spectrophotometer at a wavelength of 420nm taking the absorbance readings. In the second experiment, a phosphate buffer of PH 7.0 was used in different measures together with different measurement of potato juice and the enzyme catecholase then subjected to the spectrophotometer at a wavelength of 420nm. The data collected inform of table and analyzed using descriptive statistics such as line graph and later interpreted, showing that PH and enzyme concentration do affect the rate of enzyme reaction

Then, each tube was covered with Parafilm and inverted several times to mix the contents. Also, to each of the tubes, 30 drops of potato juice were added and covered with Parafilm and inverted several times to mix the contents. The test tubes set at room temperature for five minutes and were mixed at one minute

However one beaker received 100 mL of Deionized water with a molarity of 0.0. Afterwards a cork borer was pushed through the potato and was twisted back and forth. Once the borer was filled it was removed from the potato. Pushing the potato cylinder out of the borer, this this step was repeated six more times in order to get seven undamaged potato cylinders. Using a sharp razor blade, the potato cylinders were both cut to a uniform length of about 5cm, and were removed of their potato skins. The potato pieces were also cut in half to give the cells a greater surface area in which it was easier to absorb the solution. After the cylinders were weighed on a balance and the data was recorded in Table 4. Using the razor blade each potato was cut lengthwise into two long halves. Then the potato pieces were transferred to the water beaker and the time they were submerged was recorded. This step was repeated for all potato cylinders in which the pieces were placed in solutions 0.1 to 0.6 M. The potatoes were incubated for ninety minutes. At the end of the incubation period the time was recorded. Then the potato piece was removed form the first sample. Next potato pieces were weighed the and the final weight was recorded in Table 4. This procedure was repeated until all samples had been weighed and recorded in the chronological order they were initially placed in the test solution. Afterwards the table was completed by recording the

In this activity we will be observing the effects of potato slices and red blood cells being placed in varying molar levels of NaCl.