Investigating The Detection Limit And The Concentration Of Quinine On Tonic Water Essay

A glow stick consists of a glass vial housed inside a larger plastic vial. When you bend the stick, the glass vial breaks. This allows the two solutions to flow together and mix. The resulting chemical reaction causes a fluorescent dye to emit light according to the reaction shown on the right. The type of fluorescent dye determines the color of the glow stick.

“When light from a glowing element is analyzed through a spectroscope, it is found that the colors are the composite of a variety of different frequencies of light” (Hewitt, 2012, p. 573).

Light can be produced in many different ways, including incandescence, phosphorescence, and fluorescence (Gunderman 1). Incandescence is when heat makes an object release light, like the filament in a light bulb. Phosphorescence is when a material absorbs energy and then releases it slowly over time, like glow-in-the-dark toys. Fluorescence is when a material absorbs energy and releases it quickly as light (Gunderman 1). Fluorescence is the process used in glow sticks (Science Fair Projects 1). The fluorescent dye in this experiment’s glow sticks released yellow light. Different color glow sticks are made with different dyes (Harris 2).

When the atoms return to their ground state, they give off a color. This color is produced by the photons emitted; it has a specific amount of energy because all of the elements have a collection of different emission spectra because they all have a different amount of electrons in their energy sublevels. The energy absorbed and released has to be the same amount or it will not produce color. Only when both are equal, will a wavelength be given

Absorption spectra of chlorophyll RQ- How does different wavelengths of light affect the rate of photosynthesis? Background information - Photosynthesis is an enzyme controlled process where autotrophs convert light energy to chemical energy. This chemical energy is stored in the form of organic compounds i.e. carbohydrates. Carbon dioxide + water + light energy → carbohydrate + oxygen Photosynthesis is performed differently in different organisms, however the initial stage is always the same that is, and energy from light is absorbed from proteins called reaction centres, which contain green chlorophyll pigments, which are present inside chloroplasts. It is this organelle which allows this process to occur in certain organisms.

The fifth test tube acted as the blank test tube so was only filled with 5mL of distilled water. This tube was used to establish the minimum value of absorbency of 0. After the blank test tube was calibrated and the lowest possible value obtained, the number was subtracted by the remaining value from the measurements taken of test tubes one through four. The change in absorbency of green light in the solution of catechol and catecholase was measured using the spectrophotometer. ix.

To fully comprehend the mechanism of the bleaching process, it is essential to know the structure of the blue food dye. For this experiment, the FD&C number of the blue food dye used is Blue 1, its structure is shown as below: It could be seen that the structure has a conjugated system, which is a system of connected p-orbitals. This means that the single and double bonds alternate throughout the entire system. No electron absorbs more energy than the other because the π-orbitals overlap effectively and all π-electrons in the system are distributed above and below the molecule. The electrons are delocalized in the line of the bonds, which enables the electrons to jump between energy levels.

The spectrophotometer was used to measure the concentration of compounds in a solution according to the amount of light that passes through the solution. We warmed up the spectrophotometer for about 10 minutes and set the wavelength at 540 nm. Five test tubes were prepared each containing a different amount of catechol, water and substrate concentration. Tube one contained 1.0 mL of Buffer, pH 6.0 0.1 M NaPO4 including 0.1 mL of 0.006 M catechol, with 150 uM of substrate concentration and 1.9 mL of distilled water. Tube two contained 1.0 mL of Buffer, pH 6.0 0.1 M NaPO4 including 0.2 mL of 0.006 M catechol, with 300 uM of substrate concentration and 1.8 mL of distilled water.

The fluorescence is not really glow-in the-dark, but with specially built cameras, that block out blue light with a yellow filter, much like a swell sharks eyes it is possible to see the wonderful glowing. You are not able to see the glowing without the camera.

In this experiment we used ortho-quinone, and because it has a brown tint to it we can measure it using a spectrophotometer. In this experiment you will also need to make several dilutions. In this experiment you will take one half of the previous solution and put it in the next tube. The first solution will be full strength, the second will be ½ strength, and so on to 1/8 strength. When doing this experiment start out with twice as much full strength.

Two types of light emission are fluorescence and phosphorescence. In its stable condition, an electron exists in a ground singlet state. During a reaction, it may absorb energy and become elevated to an excited state. In most situations, the electron returns to the ground singlet state by passing through a number of vibrational states and releasing energy, which is not perceived by the human eye. However, sometimes an electron may release energy all at once in one packet to go from its excited

This experiment is about glow sticks so here are some facts. Let’s talk about glowsticks and everything about them. Light is generated from a chemical reaction which is called chemiluminescence. Typical glow sticks use a hydrogen peroxide solution, a solution of phenyl oxalate ester, and a fluorescent dye. Glow sticks are plastic tubes containing glass vials that emit light when they are cracked/ bent. They have a glow time from 6-12 hours. It also depends on the size of the glow stick and other stuff like that. Glow sticks are also waterproof if you did not already know that. Energy created by the chemical reaction is what creates the color. If glow sticks did not have fluorescent dye there would be no color. Glow sticks might look like they

In order to illustrate similar trends, the fluorescence spectra of pyrene under different detergent compounds was recorded, as shown in Figure 3. The peak of interest are observe in

Fluorescence spectroscopy is a useful technique to determine the proteins and nucleic acids of a macromolecule. Some molecules, fluorophores, can absorb light at certain wavelengths and then emit it at another wavelength. When the molecule absorbs light it is excitation and the light released is emission. The light particles, photons, hit a fluorophore to excite the molecule (Voet et. al 2013). Excitation occurs by the valence electrons of the molecule receiving energy and moving to a higher energy state from the ground state. The excited electrons will later return to the ground state because they

The first principle of quantum mechanics is quantized properties. Quantized properties are properties of particles on a very small scale This includes the principle that states that certain properties such as the location and velocity of objects can only exist in specific amounts. This principle is different than classical mechanics; classical mechanics states that these properties are continuous, and exist at more than just a specific set of quantum numbers. Quantized properties explain why there are emission lines only exist at certain