Investigating The Role Of Tcf7l2 Rs 7903146

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Objectives: The aim of this study was to investigate the possible role of TCF7L2 rs 7903146 (C/T) variant on susceptibility of T2DM among Egyptian children and adolescents.
Patients and methods: 30 T2DM pediatric patients, 20 obese children and 20 control subjects were enrolled in the study and subjected to: anthropometric measurements, routine laboratory studies including lipid profile, fasting serum insulin level and homeostatic model assessment of insulin secretion and β cell function. The rs7903146 (C/T) polymorphism was genotyped using the PCR-RFLP method.
Results: T allele of TCF7L2 rs7 903146 (C/T) was associated with T2DM in the study (P200 mg/dl in the presence of symptoms. All enrolled patients gave written informed …show more content…

HOMA-Beta=20×Fasting insulin (mU/L)/Fasting glucose (mmol/
L)-3.5 [12]. Diabetes-related (islet) autoantibody testing was used to distinguish type 1 diabetes from type 2 diabetes. Type 1 diabetes is a condition characterized by a lack of insulin due to autoimmune processes that destroy the insulin-producing beta cells in the pancreas.
Type 2 diabetes is primarily associated with insulin resistance. They included Islet Cell Cytoplasmic Autoantibodies (ICA) and Glutamic
Acid Decarboxylase Autoantibodies (GADA) using radiobinding assays to detect antibodies to in vitro transcribed and translated antigen. With type 2 diabetes, the autoantibodies are typically absent
Genotyping of TCF7L2rs7903146 polymorphism
Sample Collection and DNA Extraction: Genomic DNA was extracted from peripheral blood from, using Bo-spin whole blood genomic DNA extraction kit "Bioflux". DNA was librated, bounded to the Biospin membrane and then eluted after several washings [13].
PCR-RFLP and Genotyping: The PCR based RFLP method was employed for genotyping of the rs7903146 (C/T) polymorphism in all
PCR reactions; 0.5 mg of genomic DNA was used. PCR primers used are; forward primer (5´-ATTAGAGAGCTAAGCACTTT -3´) and backward primer (5´-GAAATGTAGCAGTGAAGTGC-3´).
PCR conditions were as follows: 5 minutes at 15°C followed by 35 cycles of 45 s at 95°C, 45 s at 56°C and final extension of 72°C for 5 min .These specific PCR primers

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