Investigating the Biomolecules found in Gelatin Essay

Carbohydrates, proteins and lipids are known as macronutrients as the body needs masses of these nutrient components in order to carry out the bodies’ essential functions.

The purpose of this lab was to test different substances using various procedures to see what biomolecules were present and ultimately find out what restaurant Anna Lyza had eaten at before she died. For the first control test, we used vegetable oil to test for lipids. So, if the solution does not contain lipids, it does not become translucent when placed onto a paper bag square and held up to a light. So, it is a negative result. However, in the presence of lipids, the solution will become translucent when placed onto a paper bag square and held up to a light. Therefore in this case, the result is positive. On the other hand, we used albumin egg to test for proteins in another control test. If the solution does not contain proteins, it will not experience any color change and so it is a negative result. When there are proteins existing in the solution, it will turn bluish/purplish and for this reason it is a positive result. Furthermore in the third control test, we used dextrose to test for simple carbohydrates such as glucose. If the solution does not contain simple carbohydrates, it will not undergo any color change and will remain a blue color. So, it is a negative result in this circumstance. If there are simple carbohydrates present in the solution, the solution will turn reddish and so the result is positive. For the last control test, we used starch solution to test

• Firstly, we used the same specimen of jelly. Although the jelly is from the same company there could be a difference in the composition of gelatine. If this was allowed to happen it could mean that it would take longer to break down some samples than others. This would then affect the overall results of the experiment as it would create an anomaly.

This Lab Report is an analysis of the results of a two-part experiment. In the first part, we used a gel filtration column to separate the components of a mixture composed of protein and non-protein molecules. By doing so we hoped to obtain fractions that contained single components of the mixture, while also gaining insight into the relative molecular weight of each component compared to each other. We would then plot these fractions onto nitrocellulose paper in order to determine which fractions had protein. In the second part, we would use the fractions which we had determined had protein to conduct an SDS-PAGE. By doing so we hoped to determine an estimate on the molecular weight of the proteins present in each fraction by comparing it to a tracker dye composed of a variety of molecules of differing molecular weight.

The purpose of this experiment was to find out the nutrient content of unidentified food samples and to use chemical reagents to see if the unidentified foods for sugar, starch, protein and fat (lipid) content. After comparing the color changes in each unknown substance then you will be able to see the amount of sugar, starch, protein or fat there is in the unknown substance.

In conclusion, this lab was a failure. Not only was the yield very small upon inspection, but the product’s composition was unknown due to the unknown solution. Given the nature of the lab, little yield was expected and observed, making the lab itself very particular in nature. Add to this the addition of the unknown solution and any produce was astounding. Not only unfortunate, the unknown solution was very dangerous. If it had been some solution that reacted violently with the reagents, there could have been unforeseen damage done to the lab and the individuals

Human error is easily obtained when working on an experiment with so many different solutions, time measurements, and accuracies of measurements. An easy mistake could have been made while waiting 15-20 minutes for the Carb Cutter to digest. With the time crunch in the Biology Lab, the Carb Cutter may not have been completely digested before rushing to begin the experiment. The results from the experiment showed an increase in absorbance with the Carb Cutter pill, which is not accurate because there is no way that more starch was made in the solution. Given more time for the procedure, this lab experiment may have received more accurate results.

2. Four unknown samples were included in the lab kit: flax seed meal, potato starch, egg whites, and dried milk. Using the results of the biochemical testing, determine which number corresponds to the correct unknown. (8 points)

J 12. F 13. C 14. K 15. K Matching—Metabolism of Carbohydrates, Proteins, and Fats 1.

7)a) You would do the Benedict’s reagent test for simple sugars and the Lugol’s solution or Iodine test for polysaccharides and starch. To test the lipids you would use the Sudan iV test and Biurets reagent test for protein.

Analysis: In this experiment, every substance tested positive for at least 1 organic compound. Corn oil tested positive for lipids. Honey and oats tested positive for starch. Honey, apple juice, and unknown 1 tested positive for sugar. Egg white, gelatin, and unknown 2 all tested positive for proteins. So, not a single substance didn't test positive for at least 1 organic compound. All the indicators used in this experiment change color when in the presence of a compound; therefore, they chemically react with the compound. No errors were made during this experiment

5. What are “lipids?” Some lipids are liquid at room temperature whereas other lipids are solid at room temperature. What accounts for this difference? Are all fats considered to be lipids? Are all lipids considered to be fats? If not, what else besides fats are considered lipids? What distinguishes “lipids” and “phospholipids?”

This lab could have contained errors. The errors could have happened when performing the lab. Some of the possible errors in this lab are:

The purpose of this lab is to identify seven unknown liquids. In class the lab group has been learning about carbs, lipids, and proteins. Carbs are the main source of energy in the human body. The elements to a carb are two hydrogen and one oxygen. Lipids are the main source of stored energy in the human body. A lipid has a lot of carbon and hydrogen and a little bit of oxygen. Proteins do all the work in the body. They make up your structure (bone marrow, collagen, muscles, and organs), they’re your immune system (white blood cells), they’re transportation (red blood cells), and they are also messengers (hormones). In the lab the dependent variable is the negative and positive results to each test. The different tests were: fat test, starch

Purpose the purpose of this experiment was to perform test to detect the presence of carbohydrates, proteins, lipids, and nucleic acids. Explain the importance of a positive and a negative control in biochemical test. Use biochemical test to identify an unknown compound.