Investigating the Effect of Lipase Concentration on the Breakdown of Fat in Milk

I put one test tube for each control, substrate, and enzyme in the 4° C (ice bath), 23° C (room temperature), 37° C (body temperature), and 60° C (water bath). Add the inhibitor that was used and what it was used for.

8) One package of active dry yeast was added to the bottle labeled ‘10 mL sugar’ and solution was swirled by rod gently.

Given the background, we hypothesized that for the first experiment, the lactase will break down lactose in milk and have a similar effect to sucrose. We also predicted that the Milk + Lactase reactant would have more glucose, the Milk + Water reactant would have a little bit of glucose broken down, the Sucrose + Lactase reactant would have less glucose than the Milk + Water reactant, and the Sucrose + Water reactant would have little to no glucose at all. As for the first procedure of the second experiment, we had hypothesized the more basic the solution would become, then the more glucose there would be. Our prediction for the first procedure of the second experiment was that there would be no glucose found in the solutions containing pH 4 and pH 7. For the second procedure of the second experiment, our hypothesis was that glucose would be present in the reactants at 4ºC and 25ºC while the reactant that had been in the hot water bath at 100ºC would have little to no glucose because it would have evaporated. We predicted that for this temperature experiment, the glucose would evaporate at 100ºC and would remain at 4ºC and 25ºC. For the first experiment we had found that a reactant of Milk + Lactase have high levels of glucose, while the other three reactants do not. As for the second experiment, for the first procedure, amounts of glucose were found in

Aim: The aim of the experiment is to test the effect temperature has on the activity of the enzyme rennin.

0.1 gram of my product from the second trial was weighed in a tray and was then added to a fourth test tube containing 2.0 mL of Iron (III) chloride, which was measured using a 10 mL graduated cylinder, to test for

6. In 1 minute intervals, we observed the color changes of the reactions and compared them to the color palette. We did this for 5 minutes.

To ensure the experiment was kept as a fair test a number of variables were controlled. The temperature of the solutions was kept constant by placing the boiling tubes into a test tube rack and setting it into a water bath with a fixed temperature of 25oC. The temperature needed to be kept low and fixed as a high temperature would denature the enzymes, they would therefore be unable to break down the gelatine and no results would be produced from the experiment. Keeping a constant temperature also meant that the solutions reacted at the same rate.

4.Measure 35mL of warm water and add them into each of the 4 test tubes at about roughly the same time. It is essential that the water is warm. Do not seal the test tube.

in all of the test tubes, some of the enzymes will work faster in some

We then placed 1.5ml of 2.5mM ONPG solution in each cuvette, creating a second serial dilution. This process helped us determine the best enzyme concentration to use for temperature, which was 1/1000. Next, we gathered eight cuvettes prepared with the serial dilution, placing two in the water bath, four in the ice, and leaving two out in the room to maintain a constant temperature. the two in the ice would be for 0 degrees Celsius, the four in the water bath will be body temperature of 37 degrees and 60 degrees Celsius, and the remaining two left out will be for the room temperature of 23 degrees Celsius. When the temperature was reached by looking at the thermometer, 1.5ml of ONPG was added and quickly placed in the spectrophotometer to determine

1 ml of water should be added to the first test tube and make a note. In the second test tube, 1 ml of methyl alcohol should be added. In the third test tube, 1 ml of hexane must be added. Lastly, the fourth test tube will be a control.

In test tube 1 add 5mL of water, 10 drops of water and 20 drops of Benedict’s solution

For this lab we will be using an enzyme to catalyze a reaction to speed up the process of turning 2H2O2 into 2H2O + O2. An enzyme is a specific protein molecule that catalyze a specific chemical reaction. The catalase enzyme will be used. Catalase is an enzyme that helps break down H2O2 (Peroxide). These enzymes can be found in your liver to help break down toxins.

The purpose of this lab is to examine the specificity of the lactase enzyme to a specific substrate and how it can denature due to the rise in temperature.

The aim of this experiment is to analyse the affects amylase inhibitors have on the rate of enzyme activity.