Investigating the Effect of Lipase Concentration on the Breakdown of Fat in Milk

1614 Words7 Pages
Investigating the Effect of Lipase Concentration on the Breakdown of Fat in Milk

INTRODUCTION:

Enzymes are proteins which can catalyse chemical reactions without changing themselves. The enzyme lipase breaks down the fat in dairy products such as full-cream milk for people who are lactose intolerant. Lipase acts on its specific substrate, lipids produces fatty acids. If enzyme concentration increases, random collisions between the substrates and active sites of enzyme increase due to the increasing amount of active sites which allow more collisions to happen, so the rate of breakdown of lipids to simpler substances will increase. During the experiment, sodium carbonate solution and pH indicator phenolphthalein will be added ahead of
…show more content…
If more were placed in one of the test tubes, then the rate of activity of the enzyme at different concentrations will be wrongly judged, leading to incorrect conclusion.

4. Temperature will be kept consistent at room temperature - All tested will be done in the Biology laboratory with the windows shut to make sure the temperature will not change during the tests. The higher the temperature, the shorter the time will be needed for the chemical reactions to happen and phenolphthalein to turn from pink to colourless.

Dependent Variable:

1. The time for the phenolphthalein to turn from pink to colourless will be taken using the stopwatch.

METHOD:

* The apparatus was collected; * The beakers labelled; Solutions of lipase respectively at concentrations of 1%, 2%, 3%, and 4% were made using lipase at 5% and distilled water. * By using the dropper and measuring cylinder, an amount of 5ml milk was placed in the test tube * By using the dropper and measuring cylinder, 10ml phenolphthalein was added to the test tube * By using the dropper and measuring cylinder, 7 ml sodium carbonate solution was added to the test tube * By using the dropper and measuring cylinder, 3ml of 1% lipase was added into the test tube * As long as the lipase was added, the button “start” on the stopwatch was pressed; and the

More about Investigating the Effect of Lipase Concentration on the Breakdown of Fat in Milk

Get Access