Investigating the effect of temperature on the action of a protease enzyme on exposed developed film

2935 Words Mar 11th, 2004 12 Pages
Investigating the effect of temperature on the action of a protease enzyme on exposed developed film

Plan

Aim: The aim of the experiment is to find out what effect temperature has on the action of a protease enzyme on exposed developed film.

Enzymes are biological catalysts. They are made in livings things built up by amino acids to make protein. Enzymes are able to speed up reactions and can repeat reactions.

There are various factors that affect the activity of enzymes they are:

"Y Temperature

"Y pH

"Y Specificity

"Y Concentration of enzyme or substrate

Enzymes are specific, this means that they only work on one substrate molecule. A substrate molecule is what the enzyme actually works on.

The factors I have chosen to
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This equipment is suitable for this experiment because it is easily available, it is easy to set up and use and it is easy to collect results with.

This is how the experiment will be set up

I will firstly measure the volume of protease solution by using a syringe, which will be 10cm3 and then put it into a test tube. I will then get two developed films and hook wire onto each so I am able to get them out of the tube easily. The wire will be labelled so it is easy to see which film is which. I will then put the test tube into an electric water bath, which is at a specific temperature for example 300C. I will leave it in the bath for three minutes and then put the two films into the test tube. Every 30 seconds I will check to see if the film has become transparent. When the two films have become transparent I take them out of the test tube. I then check the pH of the protease solution by getting a glass rod and dipping it into the solution and then put the solution onto pH paper.

Preliminary experiment:

For my preliminary experiment I set up the apparatus as above. As it was only preliminary I used one film. I chose two temperatures to put two test tubes of protease into, they were 600C and 300C. I put the two test tubes into the two different electric water baths and then after three minutes put film in each. This is how the results turned out:

Temperature of water bath/0CTest tube in water bath with no
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