Investigation Of A Titration Process

At five minute intervals over the next fifteen minute period, record the color intensity of the solution of each test tube.

1. We measured 2 mL of diluted hydrogen peroxide (the substrate), 1 mL of guaiacol (the product indicator), and 1 mL of neutral buffer (pH 7) with a syringe and disposed it into tubes 1, 2 , 4, 9, 11, and 12.

The reaction will be complete after approximately 60 seconds. Observe where color develops, and consider what these results indicate. Record your results in Table 1.

Use a test tube holder to put the test tube into a container of boiling water for 5 minutes, or until the solution changes color.

In order for the media to show the change in acidity the solutions are modified and include an indicator chemical. This indicator will change color depending on the ph level of the media it is in. For all the media used in this experiment, the indicator changes to a yellow color when in the presence of an acid and turns magenta/pink when in the presence of a base or alkali.

19. Stir the solution with a toothpick and observe any changes. Measure the pH of the new solution and record into Table 2.

After these two substances, the one measure teaspoon of calcium chloride and sodium bicarbonate, are in opposing corners of the baggie, measure five milliliters phenol red solution using the pipet and place the baggie flat on a horizontal surface.

ii. The second part of the titration series involves titration of NaOH with Hydrochloric acid (HCL). Again, three reps of titration and a blank titration have to be completed. A volumetric pipet is used to measure 10.00mL of HCL into three labeled conical flasks. Then the flasks are filled with deionized water until about the 50mL mark. A buret is

Enzyme Lab Report Jessica 9B RESEARCH QUESTION What is the effect of different concentrations of catalase on the rate of reaction? BACKGROUND RESEARCH The task of an enzyme is affected by many factors.

Take a test tube and place it in the test-tube rack. Use a medicine dropper to add 5 drops of water to the test tube. Then add 2 drops of indicator solution to the water.

By using acid-base titration, we determined the suitability of phenolphthalein and methyl red as acid base indicators. We found that the equivalence point of the titration of hydrochloric acid with sodium hydroxide was not within the ph range of phenolphthalein's color range. The titration of acetic acid with sodium hydroxide resulted in an equivalence point out of the range of methyl red. And the titration of ammonia with hydrochloric acid had an equivalence point that was also out of the range of phenolphthalein.. The methyl red indicator and the phenolphthalein indicator were unsuitable because their pH ranges for their color changes did not cover the equivalence points of the trials in which they were used. However, the

Using a suitable indicator, which changed colour over an appropriate pH range at the end point, the end point of an acid-alkali titration could be revealed. A different method of revealing the end point, which was the method that was constructed, is to continuously monitor the pH value of the solution during the titration, carrying on until well past the end point. In this experiment, there was a sudden swing in the pH value at the end point. The approximate

Depending upon the strength of an alkali and acid the right indicator is chosen: * A Titration between a strong acid and a weak alkali requires a methyl orange

Part 1 of the experiment was the standardization of the Iodine solution. First, three ascorbic acid samples were weighed out to 0.10 grams each and then placed into a 250mL Erlenmeyer flask. Each flask containing the sample was then filled with 100mL of distilled water and the ascorbic acid dissolved. Next, 100 mL of the stock I2 solution was measured into a 400mL beaker and 150 mL of water was added and thoroughly stirred. A buret was obtained, washed thoroughly, and then rinsed three times with the I2 solution. Then the buret was filled with the I2 solution. 1 mL of starch indicator was added to the first ascorbic acid sample. An initial reading on the buret was recorded and then the titration began. The flask was gently swirled to mix the solution as the titration was

A funnel will allow me to pour the acid into the burette without it spilling which will limit safety hazards.