Investigation Of An Unknown Bacteria

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Abstract: The objective of the investigation was to perform multiple experiments that would lead to the identification of an unknown bacteria. This bacteria was causing a urinary tract infection on a patient. A sample of the bacteria causing the infection was received and analyzed. The first step was to isolate the bacteria to insure that sample was free of contamination. A MAC plate, a PEA plate, along with a Nutrient agar plate where used to produce pure colonies by using the quadrant streak method on each plate. After a 24 hour incubation period the colonies where isolated. The bacteria collected was inoculated into slants, nutrient broth for cultivation. A sample from the MAC plate and the PEA plate were collected and gram…show more content…
A sample of the patients bacteria causing the urinary tract infection can only be used if a urine sample is bacteriologically viable (1 Radar). Infected urine and sterile urine are common samples used for the diagnosis of patients (1 Radar). It is important to gain further knowledge of multiple organisms’ resistance to antibiotics that cause urinary tract infections (1Radar). Escherichia coli has distinct characteristics and structures that help identify their unique properties (2 Johnson). Escherichia coli is the most common and most prevalent bacteria present in the fecal flora of the human colon (2 Johnson). Because of Escherichia coli unique ability to overcome the obstacles of a new environment, it makes easy for bacteria to spread into the urinary tract and produce a discomforting infection (2 Johnson). All research conducted lead to the belief that Escherichia coli was the Enterobacteriaceae pathogen causing the UTI in the patient. In order to confirm this prediction a set of differential tests and biochemical test must be conducted. Preliminary studies help identify Genus species of bacteria. Two different preliminary study pathways must be used since two different pathogens were found in the sample. A dilution and a quadrant streak are the ideal methods to separate pure cultures of bacteria. MacConkey Agar and CAN (MAC) is a selective media that is used for the cultivation of gram negative bacteria. (PEA) is a selective media that is used
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