Investigation of Action of Saliva and 3M Hydrochloric Acid in Two Carbohydrate Solution

789 Words Feb 5th, 2015 4 Pages
Name CHIA ZHEN YONG
Partner’s Name LIEW JUN KEAT, DUNSTAN YOUNG
Class
P1
Date of lab class 10 February 2014
Program
Foundation in science
Unit code
FHSB1214
Unit description
B IOLOGY I
Year and trimester of study
2014 , trimester 1
Title of lab report
Practical 3 : Investigation of Action of Saliva and 3M HCl in Two Carbohydrates Solution
Lecturer’s name
Cik Norkhalidah Binti Jamali

Title : Practical 3 : : Investigation of Action of Saliva and 3M HCl in Two Carbohydrates Solution
Objective : To study the relationship between two carbohydrates solution with saliva and hydrochloric acid.
Result :

Observation
Conclusion
Solution A
Benedict’s test : blue-brick red
…show more content…
Hydrochloric acid is a very strong acid and it can digest any that contact with it. At high temperature, hydrochloric acid can act as chemical enzyme which can hydrolyses polysaccharide. Hydrochloric acid can break down solution B and starch which are polysaccharide to their respective monosaccharide. Temperatures may have affect the substances such as solutions A and B, saliva, and hydrochloric acid. Saliva is a type of biological enzyme needs an optimum temperature of 37°C to carry out high activity. High temperature causes amylase to become denatured, and the shape of the active site changes, because the bonds in the enzymes are broken because of the heat energy gained to brak the bond. When an enzyme is denatured, it cannot function, hence , no catalyse biological reaction. When solution B is under high temperature, solution B gains heat, which is converted to kinetic energy. Solution B thus have high kinetic energy, and its easier to be catalyzed because the activation energy is achieved easily. Because it has high kinetic energy, the solution can be catalyzed by the hydrochloric acid.

The product that formed after hydrolysed is broken to monosaccharide which are glucose molecules. This is because glucose is a type of reducing sugar that can turns Benedict’s solution from blue to brick-red precipitate. Starch and glucose are consider as carbohydrate . Figure below shows
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