Investigation of Effect of Temperature on Amylase Activity

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Investigate the effect of temperature on amylase activity

Introduction

Amylase is an enzyme that catalyses the breakdown of starch into sugars. Amylases are found in almost all plants, animals and microorganisms. Large amounts of amylase occur in germinating cereals, and in the pancreas and saliva of higher animals.

Aim

The aim of this experiment is to find out the rate of reaction between amylase and starch in a range of different reaction temperatures.

Hypothesis

As the reaction temperature of amylase solution and starch solution increase, the reaction rate of amylase and starch will increase. After reach the optimal temperature of amylase, the reaction rate of amylase and starch will rapidly decrease.

The
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Wear safety goggles to prevent any starch solution or iodine solution getting in your eyes.

2. Some water bathes are very hot.

Don’t directly touch the water inside the water bathes. Using a rack to put test tubes into the water bathes.

3. Crushed ice can damage your hands.

Wear safety gloves to protect your hands from cold and stiff.

Method

1. Before start the experiment, lab coat, safety glasses and safety gloves must be worn. Clean up your hands as well.
2. Measure the room temperature with a new thermometer. Use marker pen to name beaker 1 and beaker 2. Divide 10 test tubes into 2 groups, name them from 1-5 in both of the group. You will need five stopwatches to record the time for the disappearance of blue-black color in test tubes, name them from 1-5 as well.Stopwatch 1 is corresponding to the test tubes 1 in beaker 1(0°C ),stopwatch 5 is corresponding to the tst tubes 5 in 80°C water bath. Record the room temperature.
3. Add crushed ice into beaker 1 and fill it with tape water. Beaker 2 only fills with tape water. Put a thermometer in both of the beakers. After a few minutes, check the thermometers in beaker 1 and beaker 2 to see if they have reached 0°C and the room temperature. Once the temperatures reach our reaction temperatures, the experiment may start.
4. Make sure that the amylase solution and starch solution you used in this experiment must have a , you may test the pH values by
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