Investigation of Whether Temperature Affects Enzyme Activity Essay

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Assessment for DCP and CE Determining if temperature affects enzyme activity This investigation is using a range of temperature in order to find weather or not Temperature affects enzyme activity. I will: 1. Observe the effects of different temperatures on enzyme activity 2. Infer the relationship between the two 3. Use a line graph to display data and draw a conclusion Research Question How does temperature affect enzymes activity? Variables Independent Variable: Temperature Dependent Variable: Time that the solution takes to become clear Controlled Variable: Enzyme Concentration, Milk Concentration, Enzyme volume, Milk volume. Background When molecules bump into each other, the kinetic energy that…show more content…
Heavy Duty Beakers 8. Thermometers 9. Black Card 10. 2 per boiling tube 11. 2 per small beakers 12. Test Tube Rack 13. Stopwatch Data Collection & Processing Recording Please see the table on the next page Calculation of the average (values of repeated numbers /4) Calculation of the rate (1/average) ×1000 Table Description On the lined piece of paper is the data that I recorder during the experiment. As you can see from the table I tested 5 different temperatures and repeated the process 4 times. This was to see the change that different temperature had on enzyme activity. My table shows some results to be a different from the others but overall these did not affect my graph’s outlook, so I chose to leave them in. Graph Description You can find my graph on graph paper. Rate in arbitrary units is on the Y-axis. Temperature measured in ˚C in on the X-axis. The graph firstly does up then decreases, reaches 0 and remains there. This is because at a certain point the enzyme becomes denatured and the activity slows down and eventually stops. Conclusion & Evaluation Concluding The results show that at first, as the temperature increases the time that the solution takes to turn clear decreases. Resulting in the rate of reaction to increase. At 65 ˚C the time taken starts to increase as the enzyme is on the edge of denaturalization. After which the enzyme no longer works and the solution stays white. When the enzyme in
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