Isolation And Identification Of Seed Borne Fungi

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Isolation and identification of Seed-Borne Fungi in Green gram Seeds A.J.Deshmukh1, A.N.Sabalpara2, V.A. Patil3 and V.P. Prajapati4

1 & 4 Asst. Professor, College of Agriculture, NAU, Waghai
2. Director of Research & Dean PG Studies, NAU, Navsari
3 Asst. Research Scientist, NARP, NAU, Navsari
Email:amol_deshmukhnau@yahoo.co.in
Abstract An experiment was undertaken to isolate and identify the seed borne fungi of Green gram. Seed samples of three Green gram varieties were collected from Pulse research station, NAU, Navsari. PDA plating method was employed for the isolation of the fungi and cultural, microscopic study was adopted for the identification. Altogether nine fungi were found in association Viz., M. phaseolina, A. alternata, C. capsici, N. sphaerica, Chaetomium sp., Aspergillus sp., R. oryzae, sterile aseptate and sterile septate fungus with all the three variety seed lots.

Keywords : Seed-Borne Fungi, Green gram, cultural, morphological study

Introduction
Green gram (Vigna radiata (L.) Wilczek) is one of the most important pulse crop of the arid and semiarid tropics (Chen et al., 1987). It is an excellent source of easily digestible protein. Several factors are responsible for low production of Green gram. Among them, diseases play an important role (Nene, 1980; Pal, 1996). Many fungal pathogens, some of which are seed transmitted, often reduce the germination ability or kill the infected plants or substantially reduce the productive capacity. However,

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