Isolation Of Caffeine From Tea Leaves Lab Report

Coffee is often used by many individuals as a stimulus to stay more alert when preforming tasks but it may not have the same effects on individuals with depression. The overproduction of the neurotransmitter Acetylcholine has been associated with people with depression. Active research have correlated high acetylcholine levels in the brain to increase depression like symptoms in healthy individuals and individuals with bipolar disorder. Other stimuli such as caffeine have been studied to determine an increase risked of depression by using dose-response methods in people who have experience depression in their lifetime. In this experiment the effects of acetylcholine and caffeine on the heart rate of a ghost shrimp, Palaemonetes, were tested to determine if the combination of these

From the results obtained from conducting this experiment, it can be observed that caffeine influences blood pressure levels, both systolic and diastolic and causes it to spike up within a short period of time. These results support the research conducted prior to this experiment, which states that caffeine consumption causes blood pressure levels to rise due to a sudden increase in adrenaline and vasoconstriction in the body.

Caffeine is a wildly use drug in today’s society. Caffeine is a methylated xanthine which acts as a mild central nervous system stimulant (MS & RL, 2001). It is a stimulant which acts upon the central nervous system and increases alertness, wakefulness and restlessness and it increases the release of catecholamine from renal medullar (Fernandez, 2016; Collines, 2007). It is present in many beverages. Caffeine is found in coffee, tea, soft drinks, products containing chocolate (cocoa) and some medication (Collines, 2007). It is used as a cardiac and respiratory stimulant (Collines, 2007).Caffeine is the most frequently ingested pharmacologically active substance in the world (Collines, 2007).

Pure caffeine is a white powder, like what is found in caffeine pills, and is “a plant-based alkaloid which

Caffeine is a mild stimulant that occurs naturally in at least 63 plant species. Caffeine can be found in the leaves, stems, seeds, and roots of these various plants. Caffeine is part of the methylxanthine family. It consists of a xanthine molecule with three methyl groups attached to it. Caffeine can be found in many products like sodas, tea, and coffee, but it also occurs in several other products such as prescription medications, diuretics, and pain relievers. Caffeine’s widespread use and popularity have caused many people to view the substance as an addictive drug. Thus making caffeine the most inexpensive and readily available drug known to man. Then on the other hand there are

To measure baseline heart rate, single Daphnia magna was placed into a well of a concave slide and excess water removed using a tissue. A coverslip was not used as it would injure the specimen. The water was enough for gills to function normally with the specimen immobilized.

When the crude product is transferred to a separatory funnel, it is washed with 10 ml of water. When the solution forms two layers, the bottom aqueous layer is disposed of.

5.    Prepare the caffeine solution by dissolving 10g of caffeine tablets in 100ml of water in a beaker. Label the beaker ‘caffeine’. Similarly, add 10g of coffee to 100ml of water in another beaker and label it ‘coffee’.

Caffeine is found in many plant species, where it acts as a natural pesticide. It is found most commonly in cocoa, tea and coffee, but is also artificially added to some soft drinks such as cola to act as a flavour enhancer. When consumed by humans, caffeine works as a stimulant causing amounts of released neurotransmitters to be increased. High use of caffeine has been related with raised blood pressure, restlessness, insomnia and anxiety which, in the long term, can lead to heart and circulation problems.

Caffeine is an odorless, slightly bitter solid. It is a stimulant. Caffeine dissolves in water and alcohol, and has crystals that look like needles. When taken in small

In this experiment, the purpose of this experiment is to isolate pure caffeine from tealeaves. Caffeine does not exist alone within the tealeaves, but other substances that are in the leaves itself accompany it. There are major and minor products that make up the constancy of the leaves and with those substances they need to be separated out of the leaves. The main component of the leaves are, cellulose and polymer of glucose, while the minor components of the leaves are caffeine, tannis, flauaniods and chlorophylls. By weight, caffeine is 5%, which is an alkaloid and contains C, H, N, O; it is very soluble in water and is the main component that will be extracted from the experiment. Although caffeine is very soluble in water it is much more soluble when it is dissolved in methylene chloride. Therefore, when completing the methylene chloride process, it will nearly purify all of the caffeine, making it pure caffeine. Make sure not to breathe in the fumes of the chemicals or spill them on yourself. Safety precautions

The objective of this experiment was to isolate caffeine from tea bags and purify the caffeine by a sublimation process. To accomplish this a liquid-liquid extraction technique was used along with the process of sublimation. During the liquid-liquid extraction the sodium carbonate deprotonates the caffeine so that it is more soluble in the organic layer. The organic layer is colorless because it contains the caffeine while the dark colored tannins move into the aqueous layer. Sublimation is the process by which a solid transitions directly into the gas phase.

Components containing caffeine were composed into stock solutions. These solutions were diluted to 1: 10 substance: mobile phase. A stock solution of caffeine was diluted 1:50. A sequence of diluted caffeine solutions were prepared for use as a standard (ppm): 1, 2, 4, and 10. Solutions of acetaminophen, acetylsalicylic acid, and Goody’s Powder were developed to differentiate chromatographic peaks observed. These solutions were subjected to HPLC for examination of the observed peak area and retention time for the set of compounds. Comparison of retention time allowed for the differentiation of peaks observed. The peak area obtained was utilized to determine the relative concentration of caffeine present in Goody’s Powder based on the relationship obtained in the standard. The content of caffeine present in Goody’s Powder by percent weight was identified.

In the solid-liquid extraction all water soluble components were sepreated along with the caffeine including the tannins. To further isolate the caffeine and obtain a purer product it was necessary to add sodium carbonate(Zhao et al. 208). This acidic- base reaction (tannins being the acid electrophile and sodium carbonate the base nucleophile) forms conjugate base phenolic salts. Tannins are acidic and thus have an–OH group directly bound to their aromatic ring the addition of a weaker base allows for the deprotonation of said –OH group. Then when the methylene chloride was added the tannins, now a phenolic salt, were insoluble thus resulting in the formation of two layers (organic and aqeous). The aqeuous layer now contains the tannins and chlorphyll and the organic layer the caffeine. This occurs because caffeine is more soluble in the methylene chloride and thus is separtaed into the same layer and the tannin salts and chlorophyll which are insoluble in the methylene chloride remain in the aqeous layer. The organic layer is extracted and undergeos this same washing process again in order to extract the maximum amount of product. Once enough caffeine was extracted a drying agent of anyhydrous sulfate was added to remove any of the excess water in the organic layer and placed in a hot chamber to evaporate off the methylene chloride. The remaining crystals underwent recrystalization to obtain a pure substance. (Zhao et al. 208).

1. According to the Merriam-Webster dictionary caffeine is defined as a bitter alkaloid C8H10N4O2 (not that any of you care) found especially in coffee, tea, cacao, and kola nuts and used medicinally as a stimulant and diuretic