Isolation of Caffeine from a Tea Bag

According to the results, the columns of caffeine in figure 1, of this experiment the hypothesis for caffeine is partially accepted. There is an increasing trend in the change of pulsation rates with increasing

Many manufactures release the caffeine content of their products publically, but not always, and new products and flavors are continuously introduced to the market. If quality checks are not performed, manufactures may alter the caffeine and benzoic acid content to suit the demands without public knowledge. To ensure the levels of caffeine and benzoic acid in products do not exceed the established safe limits and to inform the public of the amount of these compounds being consumed, various methods of analysis have been performed. Before the introduction of modern techniques, spectrophotometric methods alone were used to determine concentration of a compound in a mixture.6 The caffeine content in coffee, tea, soft drink, and energy drinks were determined using an immunoassay.7 The caffeine content in mixtures also used to have to be extracted before quantification.8

Pure caffeine is a white powder, like what is found in caffeine pills, and is “a plant-based alkaloid which

12) Pipet the remaining methylene chloride to a new beaker. Place the flask in hot water bath, and boil the methylene chloride out, leaving the benzoin and the dibromobenzene .

This science fair project was performed to find out the effect on plant growth of adding caffeine to the soil. The experiment was done by using mung bean plants and watering them daily with water, water mixed with caffeine and with a coffee mixture.

7. Using the slider on the right hand side, add NaOH to the HCl in the Erlenmeyer flask (This action is known as titrate). Add the indicator until the color of the indicator turns a light shade of pink.

Thesis: Caffeine can have many different effects on the body depending on the amount of consumption.

Caffeine is an odorless, slightly bitter solid. It is a stimulant. Caffeine dissolves in water and alcohol, and has crystals that look like needles. When taken in small

Components containing caffeine were composed into stock solutions. These solutions were diluted to 1: 10 substance: mobile phase. A stock solution of caffeine was diluted 1:50. A sequence of diluted caffeine solutions were prepared for use as a standard (ppm): 1, 2, 4, and 10. Solutions of acetaminophen, acetylsalicylic acid, and Goody’s Powder were developed to differentiate chromatographic peaks observed. These solutions were subjected to HPLC for examination of the observed peak area and retention time for the set of compounds. Comparison of retention time allowed for the differentiation of peaks observed. The peak area obtained was utilized to determine the relative concentration of caffeine present in Goody’s Powder based on the relationship obtained in the standard. The content of caffeine present in Goody’s Powder by percent weight was identified.

Remove foam plug from the inoculate flask and pour it into a cuvette. Incubate it at 37°C and the optical density readings at 600nm every 10-15 mins. Pour into the

One on the beneficial effects on caffeine, two the negative effects, and finally on trends in caffeine consumption

Many of us find ourselves drinking a freshly brewed cup of coffee, an ice-cold soft drink, a hot cup of tea, or an energy drink whether it be in the morning, afternoon, or in the night but one thing is that they all share a common ingredient: Caffeine

Ever stay up late studying for a test or exam and wake up exhausted? Or just experienced a long day and need an extra boost? Many people believe caffeine is the answer to anyone’s drained state of being. Healthline.com explains that, “Caffeine reaches your brain quickly and acts as a central nervous system stimulant.” Caffeine is commonly referred to the most widely used drug on the planet, so the next question is which caffeine-filled product is the most efficient? It is widely debated whether energy drinks or coffee can provide the best balance of caffeine, flavor, and other ingredients, but after researching, I have come to conclude coffee is superlative in all aspects.

The ingredient most attractive to buyers of the drink is 1,3,7,-trimethylxanthine or more commonly, caffeine. Caffeine is a purine: a heterocyclic organic compound , in which a pyrimidine ring is attached to an imidazole ring, sharing a carbon-carbon double bond. Figure 1 illustrates the structure of a caffeine molecule, which has molecular formula C8H10N4O2. Caffeine consists of the following functional groups :

1. Place the beaker containing the mixture on the hot plate and heat until the white fumes no longer form (about 15 minutes) during the sublimation process.