Isolation of Individual Colonies

Purpose: To learn about the international system of units (SI), to become familiar with common lab equipment and techniques, to gain proficiency in determining volume, mass, length, and temperature of a variety of items using common laboratory measurement devices, to learn to combine units to determine density and concentration, and to use laboratory equipment to create serial dilutions and determine the density and concentration of each dilution.

Procedure: I used a ruler, thermometer, and scale to take measurements. I used a graduated cylinder, short step pipet, scale, and ruler to determine volume and density. I used a volumetric flask, graduated pipet, pipet bulb, scale, and glass beaker to determine concentrations and densities of various dilutions.

7- what term refer to allowing food to remain for too ong a time in a temperature that allows the growth of germs

Me and my lab partner, obtained a mixture of a un known proportion from the instructor and then flow the guide line in our lab manual to separate the mixture by applying the separation method motioned in our lab manual pages 33-40 . In this experiment, the separation methods were decantation,

• Serially dilute the 4 mg/ml solution with buffer A to make working solutions of 400 µg/ml and 40 µg/ml.

0.1 gram of my product from the second trial was weighed in a tray and was then added to a fourth test tube containing 2.0 mL of Iron (III) chloride, which was measured using a 10 mL graduated cylinder, to test for

Our purpose in this lab was to create four solutions of the same components and different concentration. I found the concentration in Molarity (mol/liter). I would dilute a solution to create new solutions, using the formula M1xV1=M2xV2 where M=molarity and V=volume. In this lab, there were four solutions, Solution A, Solution B, Solution C, and Solution D. The solute or substance that dissolves was sugar and the solvent or substance that the solute dissolves in was water. For Solution A, I weighed 3.42 g sugar and diluted to 100 mL to get a concentration of 0.10 M and the color of bright red. For Solution B, I weighed 0.342 g sugar and diluted to 100 mL to get a concentration of 0.010 M and the color of pink. For Solution C, I measured 10

We placed 50 mL of vinegar, tap water, seawater into three respective beakers. 50 mL of seltzer water was placed into a jar with a lid

1. Gathered all required materials to designated lab bench. 2. Considered all safety precautions including the prevention of spilling water to avoid falls, handling glassware carefully to prevent shattering, avoiding long periods of working with warm water to avoid burns and avoiding the digestion/inhalation of by-products produced after the reaction (e.g. ethanol and carbon dioxide gas). 3.

The purpose of this lab was for the student to get involved with his or hers new lab kit as well as being able to know, identify and use each other tools provided in the kit. Another key learning aspect of this lab is to teach the student how to measure properly the many units in the SI system. I will be using laboratory dilutions, measurements, and weights to then calculate using algebraic formula.

The purpose of this lab is to see the effects of pasteurization while emphasizing the process for serial dilutions.

Preparing serial dilutions of the alfalfa water will ensure an accurate count. The plates are incubated until you see visible colonies, usually 18-24 hours. The colonies you see growing on the plate are considered to have started from one viable bacterial unit but because bacteria are usually not found as individuals, the colony you see may have started from a single cell or a group of cells. The results are reported as colony forming units (CFU’s).

and the number of False Negatives. In another way it is the number of positive

PBS and trypan-blue are required for carrying out the cell counts with a Hemocytometer. The cell suspension is transferred to a chamber on the hemocytometer and a microscope is used to identify the number of trypan-blue excluding cells,

Abstract- In medical analysis blood cell count plays vital role. Variations in the count of blood cells cause many diseases in the human body. For overall health assessment and diagnosis of many disorders complete blood count is required. Abnormal increase or decrease in cell count indicates that person has indispensable medical condition. The Complete Blood Count (CBC) is a blood test, extensively used to check various disorders such as infections, allergies, problems with clotting, anaemia, leukaemia etc. In order to perform CBC test, the blood film is stained and then imaged with a transmission light microscope. Here the analysis of the blood sample is done manually in order to count number of blood cells and also to identify disorders in blood samples through a microscope. But it is a time consuming process and also leads to undesirable human error. In essence, the goal of this review paper is to find out and validate the necessary image processing steps and different methods and algorithms used to count blood cells on blood smear slides. This paper aims to provide: mitigate problems posed by different conditions such as noisy and degraded images; detect the overlapping cells; to differentiate RBCs ,WBCs and also platelets which are present in a blood smear slide counting RBCs and WBCs and even platelets and also to detect the disease related to blood.