Contents ANTIMICROBIAL PROPERTIES OF SOIL 2 ABSTRACT 2 LITERATURE REVIEW 3 AIM OF RESEARCH 4 OBJECTIVES OF STUDY 4 RESEARCH METHODOLOGY 4 METHOD(S) TO FACILITATE MICROBIAL IDENTIFICATION 5 Gram stain 5 Culturing 5 MATERIALS TO BE USED 5 ETHICAL CONSIDERATIONS 6 SAMPLE COLLECTION 6 SAMPLE ANALYSIS METHOD 7 DAY 3 – Expected Results 9 DISSEMINATION OF
Antimicrobial Activity of Soil Isolates Abstract: Isolation and characterization of microorganisms is a practice that aids in Increasing ones knowledge of a laboratory setting and it helps improve on Using sterile technique. Isolates of soil microbes can be categorized and Characterized based on a number of criteria ranging from gram-staining Which is done for this project to enumeration which is quantitative description Based
specific requirements for growth (Benito, 2012). Bacteria can be grown in both liquid and solid medium, as they contain the nutrient that is needed for growth. They also provide the required moisture and pH to maintain bacteria growth. Bacteria cultures are incubated at optimum temperature for proper growth. The bacteria (micro flora) that live on the body surface are mesophiles- they grow at tempertures between 25°C and 45°C (MacConkey, 1905). The medium used for this experiment was Nutrient agar
Phase I-Phenotypic characterization 1. Collection of microbial isolates from various sample, characterization, identification and performing antibiotic susceptibility testing. 2. Performing Metallo Beta Lactamase (MBL) screening and confirmatory tests. 3. Testing carbapenemase production phenotypically. 4. Testing microbial isolates against higher and multidrug antibiotics. Phase II-Molecular Characterization 1. To identify the type of carbapenemase and MBL genes by PCR 2. To identify the expression
spread plate isolation. Isolated bacteria were subject to colony characterization and were estimated by their morphological and biochemical characters. As being a monsoon the occurrence of variation of different species were high. The microorganisms isolated from the soil were of staphylococcus strain and were gram positive, aerobic, coccus shaped
milk,establishment of pure cultures of LAB,identify LAB and phage recovery and enumeration of recoverd phage.Raw milk was chosen as a sample so as to have a more positive result.To identify bacteria Lab isolated from raw milk,biochemical,morphological ,physiological and cultural characteristics were employed. The purification of isolates was done by moving Gram +ve ro ds and cocci shaped bacteria to selective media MRS and M-17 plates. The isolates were sub cultured till pure
URINE ANALYSIS ....................................................................................................13 3. STOOL TEST FOR OVA AND CYST ......................................................................15 5. CULTURE AND IDENTIFICATION OF BACTERIA .......................................................17 1. GRAMSTAINING (PRIMARY IDENTIFICATION) ...............................................20 2. BIOCHEMICAL TESTS (SECONDARY IDENTIFICATION) ........
1.0 INTRODUCTION Ready to eat (RTE) foods can also be described as food raw or cooked, hot or chilled that is ready for immediate consumption at the point of sale without further treatment (Tsang, 2002). There are certain appealing factors that make street foods popular as food sources; familiarity, taste, low cost and convenience (Mahakarnchanakul et al., 2010). Our society shows a social pattern characterized by increased mobility, large number of itinerary workers and less family or home centered