Isopod Lab Report

Pillbugs and sowbugs are terrestrial isopods that belong to the order Isopoda and the arthropod class crustacean. According to the Current Topics in Microbiology and Immunology, the terrestrial isopods have evolved from the marine forms. However despite the fact that they seek moist environments they must also avoid an overly moist environment (Journal of Insect Science, 2008). The two different species resemble one another phenotypically. However, pillbugs are a dark gray and the sow bugs are a light gray with posterior projections. Pillbugs also curl into a ball when they feel endangered and the sow bugs do not. The pill bugs used in this experiment were female. Isopods primarily live in dark, damp habitats in order to prevent dehydration (Isopod Behavior Lab). The purpose of this experiment was to determine the type of environment isopods prefer. First we tested to see if the isopods preferred a dry or moist environment and then we tested to see if they preferred the potting soil or the leaf litter environment. For the observation of isopods in response to moisture experiment it was said that each of the two different species would prefer a different environment. For part two, Observation of Isopods in response to other variables, it was hypothesized that the Isopods would prefer the potting soil or the leaf litter environment.

The mole is a convenient unit for analyzing chemical reactions. Avogadro’s number is equal to the mole. The mass of a mole of any compound or element is the mass in grams that corresponds to the molecular formula, also known as the atomic mass. In this experiment, you will observe the reaction of iron nails with a solution of copper (II) chloride and determine the number of moles involved in the reaction. You will determine the number of moles of copper produced in the reaction of iron and copper (II) chloride, determine the number of moles of iron used up in the reaction of iron and copper (II) chloride, determine the ratio of moles of iron to moles of copper, and determine the number of atoms and formula units involved in

The isopods used for this experiment range in color from gray to brown and were about 8.5 to 18 mm in length. Each Isopod’s head had one pair of antennae and one pair of antennules, both used to detect sensory stimuli from the environment around the pillbug. Their bodies were made of a hard thorax with seven segments and an abdomen. Each pillbug had seven pairs of legs, one for each segment of its thorax. Pill bugs breathe through gill-like structures and must live in moist places.

Rubisco is the most abundant protein on earth that is essential for carbon fixation in plants. For the protein to function at its optimal level, it needs to be isolated from the mixture of proteins and in its purest form. The three isolation techniques carried out in this lab are salting out, ion exchange chromatography, and SDS-PAGE. Rubisco will be purer as each technique is conducted and will be in its purest form after the last isolation technique is carried out.

Introduction: The biological membranes are composed of phospholipid bilayers, each phospholipid with hydrophilic heads and hydrophobic tails, and proteins. This arrangement of the proteins and lipids produces a selectively permeable membrane. Many kinds of molecules surround or are contained within

Ionic compounds are soluble in water to a certain point depending on the compound. The level of solubility changes among different compounds. Some ionic compounds can completely dissolve in water and appear to be a homogeneous mixture. Although, some ionic compounds dissolve very little, and could be considered insoluble, since it does not dissolve fully. Depending on the compound, the level of solubility can be high or low. However, ionic compounds could dissolve to a certain degree. If the solution appears to be a heterogeneous mixture, many may assume through visual representation that it may be insoluble. As stated previously, the smallest amount of solubility should be considered. To confirm whether or not the substance is soluble, observe the efficiency when conducting electricity. Due to practical reasons, the slightest solubility could be considered insoluble by people.

The atom of an element has electrons that are found around the nucleus in regions known as orbitals. When energy is absorbed by the electrons of an atom they begin to jump to higher energy levels. When this happens the electrons are in an excited state. However when the electrons begin to release the energy and drop in energy levels they emit electromagnetic radiation. If the radiation that is emitted falls between 400 to 700 nanometers then the electrons emit photons which we can see as visible light.

The oxidation number of an atom of any free element is ZERO. Means to say there is only one kind of atom present, no charge.

Begining by labeling 7 different 2.0 ml tubes 0 thru 6 for each compound. Then add 1ml of extract to tube 0. Then add 0.5 ml of DMSO to tubes 1 thru 6. Now make a 1:2 serial dilution from 0(pure extract) to 6(1:16)

Isopods are land dwelling crustaceans also known as pill bugs. In the lab the isopods will be studied on ethology which is the study of animal behavior. The taxis and kinesis will also be studied of the isopods. Taxis is when a certain factor attracts or repels an individual. Kinesis is when an individual randomly moves because of a certain factor.

Why do organisms respond to environmental factors? Organisms keep themselves in the most favorable conditions in order to survive. Pill bugs are members of the terrestrial isopod family. Terrestrial Isopods retrieve oxygen through their gills and live in dark damp environments. These organisms eat decaying plant and animal matter. Sow bugs, also known as pill bugs were tested to see how they react or respond to environmental factors. In this lab two different experiments were conducted. During the first experiment pill bugs were tested to see if they prefer a light environment or a dark environment. Throughout second experiment the pill bugs were tested to see how many of the bugs prefer an environment with vinegar or environment

All living things respond to stimuli, including animals. In our experiment, we tested how roly-polies, or pill bugs, respond to two types of material: wet sand and wet dirt. Pill bugs are isopods, a group of 10,000 species living on land and in fresh water and ocean. They are under the phylum Arthropoda, class Crustacea, containing both crabs and shrimp. Pill bugs generally live in dark, moist environments with the decaying matter they eat.

2. (5 pts) List and explain the names and affiliations of the various characters/stakeholders in this story – I’m looking for us to use the story to map out the complexities that are generally associated with solving public health puzzles – the stakeholders you list and explain here should apply to many of the cases we consider going forward.

If I turned over a rock and find isopods under it, they would most likely be crawling around. However, they would all go in the ground for protection if I continued to watch them for a few minutes.

Introduction Proteins are critical components in understanding cells and organisms which can contribute to further developments in medicine. Proteins compose more than 50% of the dry weight of cells [2]. Enzymes are the proteins that catalyze most reactions in a cell that keeps the cell going. By isolating protein scientists can understand, modify, and sequence specific proteins away from other cellular components. The specific protein being looked at is Rubisco (Ribulose-1,5-bisphosphate carboxylase/ oxygenase) which is a plant enzyme that has a key role in photosynthesis [1].