Isotonic Solutions Effects On Living Cells

6. What results would you expect if the experiment started with glucose and Lugol’s Solution

Explain what happened to the blood cells at the various levels of concentration. Be sure to refer to the solutions as being hypotonic, hypertonic and isotonic.

The experiment is done to visualize tonicity and osmoregulation in a mock animal cell for comprehension of the concept. A grape will be used for the representation and exposed to three different tonicities. The changes or lack thereof in weight can show the movement of water to balance its tonicity through osmoregulation. The predicted results with the green grapes in solution after four days of research will be reflective of how a hypotonic, hypertonic, and isotonic cell will react. The hypotonic solution will cause the grape to shrivel noticeably and be slightly browned, leaving it with less weight. The hypertonic solution will cause the grape to have the outer skin torn, starting from the where the stem attached, the grape should be expanded as well. The weight will increase. The isotonic solution will have little change, still plump and the same weight.

In this lab experiment, half our group observed and measured osmosis using dialysis tubes that were represented as the semipermeable membrane. It is permeable to water and other small molecules but is impermeable to larger molecules such as the sucrose solution used in each of the four beakers and tubing. The other half of our group observed the tonicity of sheep blood to determine whether the blood was isotonic, hypotonic, or hypertonic. The 85 g/dL of NaCl solution was the ideal isotonic number in relation to the sheep blood cells as well as a reference to the other observations of the solutions.

Purpose: The purpose of this lab is to familiarize you with osmosis and, specifically, what happens to cells when they are exposed to solutions of differing tonicities.

The main focus of this lab was to measure different tonicities. Tonicity is the concentration of the solute particle mixed with the solvent inside the cell versus said the concentration of solute and solvent outside the cell (Mader 50). There are three different types of tonicity measurements. One type of tonicity is called isotonic. This means that the solution has an equal concentration of solute and solvent both outside and inside the cell (Mader 50). This means that the concentration is in an equilibrium. The second tonicity is called hypertonic. This type of solution has less free water outside of the cell and a higher concentration of the solute particle. Water exits the cell in order to balance the concentration inside and outside the

Table 1 shows the contents of the bags and the content of the concentration it was submersed in. Bags 2-4 each contain a solution of both sucrose and water. These bags were each put into beakers containing hypertonic solution. These bags gained weight over time because the water moved from its high concentration inside the beaker to the low concentration inside the membrane of the artificial cell, the membrane being the bags that consisted of dialysis tubing. The

Finally, it could also be a hypertonic solution which is when there is a higher concentration of solute in the solution than in the cell and therefore the water leaves the cell. This make the cell plasmolyzed or “shrunken”. In our experiment this means that the potato cell would

The hypothesis states that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant. In order to test the predictions of the hypotonic, hypertonic, and isotonic hypothesis for the solution  made during the study,  four samples of sucrose were taken and placed into two different beakers each containing a different concentration. Then dialysis tubing A was placed into beaker 1 with B, C, and D placed into beaker 2 for 45 minutes and weighted at 15 minute intervals. My finding in the study was that each of the four samples changed from their initial weight and for the most part accurately proved the hypothesis.

In a cell, diffusion occurs across a selective permeable membrane of the cell that allows the cell to regulate and control the passage of substances. As well as controlling what passes through the membrane, the selective permeability also controls how quickly those allowed substances are able to pass through the cell membrane. When a cell is placed in a hypotonic solution, the substance wants to quickly diffuse through the cellular membrane in attempt to balance the concentration. Due to this, too much of the substance enters the cell causing the cell to swell and rupture, otherwise known as hemolysis. The purpose of this lab is to test if and how the factor of polarity of a molecule affects the diffusion rate of those molecules through a permeable red blood cell membrane in a sample of ovine blood. Testing this factor and its effect on diffusion rate is important in the biological world as it helps understand how our body regulates our own substances and molecules within us, but also how our body will react and respond to other substances such as medicines. It is hypothesized that this factor of polarity will determine how quickly or slowly molecules will diffuse across the cell membrane. Smaller molecules that have relatively low polarity will be able to cross the membrane more easily and at a faster rate than those molecules that

I predicted that as more concentrated salt solution added to the potatoes, it will cause the mass of them to decrease. because as higher concentration of sault solution cause water to move out the cell. The reason being is because water moves from a hyperosmotic area to (more concentration) a hypsometric area (less concentration).

what happens when a cell is placed in isotonic, hypertonic, and hypotonic solutions ; why an egg without it s shell is a good model of showing osmosis across a cell’s membrane and why , in order to perform the experiment, the egg needs to be soaked in vinegar first ; describe the concentrations of the solute to solvent in vinegar, syrup, and water ) .

The objective of this lab was to build a selectively permeable membrane and observe osmosis and diffusion through this membrane. There were four cells created; starch, starch and amylase, salt and glucose. For testing if starch was apparent a few drops of iodine where placed in each cell. For all of the cells starch was not apparent this is because starch molecules are too large to pass through the cell

Hypothesis: If the salt solution concentration is increased, then the cell’s vacuole size will decrease.

In this experiment I am going to investigate the effect of varying concentration of a differing glucose solution on the amount of osmotic activity, between the solution and a potato tuber of a given size. The purpose of this experiment is to demonstrate how living cells rely on osmosis, the diffusion of water.