Lab 04 - Chromatography and Ionic vs Covalent Bonds

1417 Words Jan 30th, 2015 6 Pages
Student Name: Melissa Tatum
Student ID: 4593119
Date: 7 Dec 2014
Course and Section Number: SCIN131 A004 Fall 14

Lesson 4 Lab: Chromatography and Ionic versus Covalent Bonds


Begin by viewing the following Thinkwell video

15.1.3 CIA Demonstration: Chromatography

After you watch the above video, answer the questions below in sufficient detail:

(a) (3 pts.) This video discusses 3 different types of chromatography. List each one mentioned, and describe their differences in as much detail as possible (your points earned will be proportional to the level of detail in your discussion). Which one was used in this lab demonstration?

Answer: Gas chromatography (GC) – utilized by scientists in order to be able to separate the volatile
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The stationary phase will absorb or slow down different components of the tested solution to different degrees creating layers as the components of the solution are separated. Chromatography was invented by the Russian botanist, Mikhail Tsvet. Chemists use this process to identify unknown substances by separating them into the different molecules that make them up.

(c) Suppose I melted a grape (purple) popsicle and ran a chromatogram of the resulting substrate.
[i] (1 pts.) In the simplist case, what would I expect to see? Include as much detail as possible.

Answer: Once the chromatogram has been completed and is ready to be measured and calculated, on the plate that was used to perform the chromatogram you should see where the red and blue have completely separated. The red food coloring dye should be lower on the plate than the blue food coloring dye.
<- example of results

[ii] (4 pts.) Suppose that the dot/area representing the longest wavelength of color in this situation was located 21 mm from the baseline, and the dot/area representing the shortest wavelength of color was located 36 mm from the baseline (see Figure 9.4 on page 288 for help). If the solvent traveled 57 mm from the baseline, what was the Rf for each of these two components of the sample? [SHOW ALL WORK TO RECEIVE CREDIT]

Answer: Rf = distance sample moved from

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