Lab 1 again Essay

756 WordsNov 4, 20144 Pages
Enzyme Kinetics and Protein Determination: How Enzyme Catalase Concentration Affects Reaction Rate and Determining the Identity of Unknown Proteins through Absorbance By: Alexander Mak 7238991 Partner: Yasmin Ismail BIO2137 Section A7 Corrector: Chieu Anh Ta September 18, 2014 Introduction: The first lab's primary objective is to observe the different reactions rates amongst the five different catalse concentrations of parsnip. The rate at which the enzyme catalyzes increases in relation to the increase of concentration within the test tubes. By increasing the concentration per total volume, the reaction ultimately increases the amount of contents reacting per second while decreasing the overall activation energy. The…show more content…
The parallel of the two variables show a clear link. The greater the concentration of catalase stock was less diluted (more enzyme than water), you can observe a higher rate of reaction and subsequently a faster rate. Consequently, you see as the greater the enzyme is diluted with water, a lower rate of reaction and the longer the reaction takes to occur. The higher the enzymatic contents are in the test tube, the higher the rate of reaction. Part 2 U1-K: Absorbance = 0.382 U2-Q: Absorbance = 0.697 Y = 0.2091x - 0.215 R² = 0.9527 0.382/0.2091 -0.215 =x= 1.612mg/mL = Concentration of U1-K 0.697/0.2091 -0.215=x=3.118 mg/mL = Concentration of U2-Q Discussion: Part 1: These are the expected results to be found in this type of reaction. The results are in concordance with my hypothesis as the rate of reaction depends on the amount of enzymatic contents there are or lack thereof. We are then able to predict based on the outcomes we have collected the rate it would take different concentrations of catalase to react. If the concentration of the catalase was increased to 100%, you would expect to see an increase in reaction time and an increase in the graph curve. The Part 2: It is important to use the linear part of a standard curve because the optical density measurements will usually form a straight line when
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