Lab 6: Cellular Respiration

In this lab we are measuring the amount of oxygen used in both germinating and non germinating peas. We are measuring the oxygen consumption by taking a reading of a respirometer submerged in two water baths. The first bath will be cold water and the second warm to determine the effect of temperatures on oxygen consumption. Our negative control will be glass beads to measure to increase or decrease in atmospheric pressure or temperature changes. There is a direct relationship between oxygen consumption and Carbon Dioxide produced, therefore the more O2 consumed the more CO2 produced. To keep the amount of CO2 produced from canceling out any pressure gained or lost from the consumption of

To performed the experiment, a volumeter was set up to measure the net oxygen production under white light, then a second step was followed to measure oxygen consumption under dark conditions (oxygen production only happens in the presence of light and oxygen consumption in the presence of dark light) and finally, a third step consisted of recording the measure of the net oxygen production under the presence of green light.

Figure 1: Percentage change in potato tuber mass vs. sucrose concentration. The percent change in mass decreased as the sucrose concentration increased. Relative osmotic concentration was measured as the percent change in mass of sucrose concentration over one hour.

The rate of photosynthesis can be determined different ways. Because oxygen is a product of photosynthesis and the Elodea plant is submerged in water, the oxygen is released in bubbles that rise to the surface of the water in the beaker. In this experiment, the rate of photosynthesis for each degree of light intensity can be measured by counting the number of bubbles released every 30 seconds for five minutes at each distance. The rate is the number of bubbles released per minute.

There are many procedures during this lab and many materials needed for an accurate analysis of data. First, fill a 100 mL graduated cylinder with 50 mL of water. Add 25 germinating peas and determine the amount of water that is displaced. Record this volume of the 25 germinating peas, then remove the peas and put those peas on a paper towel. They will be used for the first respirometer. Next, refill the graduated cylinder with 50 mL of water and add 25 non-germinating peas to it. Add glass beads to the graduated cylinder until the volume is the same to that of germinating peas. Remove the beads and peas and put on a paper towel. They will be used in respirometer 2. Now, the graduated cylinder was filled once again, determine how many glass beads will be require to reach the same volume of the germinating peas. Remove the beads and they will be used in respirometer 3. Then repeat the procedures used above to prepare a second set of germinating peas, dry peas and beads, and beads to be used in respirometers 4,5,and 6, the only difference is the temperature of the water.

Cellular respiration involves glycolysis, the Krebs cycle, and the electron transport chain. Glycolysis is a

The results of the mean of the concentrations are as followed; 0%, 0.2%, 2.0% and 1.0% which are in organization of longest float time to shortest. The higher the concentration of sodium bicarbonate (carbon source) the quicker the process of photosynthesis occurs. The mean of the concentrations is; 0% is 900, 0.20% is 757.58, 1.0% is 580.004 and 2.0% is 582.54. The standard deviations of the concentrations are; 0% is 0, 0.20% is 190.0045, 1.0% is 247.9306 and 2.0% is 199.979.

The hypothesis that red light would produce more dissolved oxygen was not supported by the results of the experiment. Without having more accurate data it is impossible to say which color light induced its plant to produce the most oxygen. The reason for not having more accurate data is because of the way of testing the dissolved oxygen. By testing with the dissolved oxygen tabs the data could not have been very specific and specific data is important for a good experiment. The way of getting much more accurate data is by testing with the Winkler's method. This way of testing for dissolved oxygen amounts would have not been possible to execute because of the amount of water and the size of tanks used. To test with the Winkler’s method it is necessary to test 200 mL at a time. If this way of testing for

The oxygen levels demonstrated a slightly different graph than the temperature. The oxtengen levels started at 11.84 mg/L. This saturation level rose for the next 2 meters peaking at 11.6 mg/L. Within a few meters the oxygen level dropped to 5.1 mg/L at 5 meters deep, at 6 meters the oxygen

In conclusion to the experiment, it was discovered that all of the substances being tested showed respiration except for the non-germinating peas. The trends in the most of the graphs showed the level of oxygen decreasing while the amount of carbon dioxide increasing. In comparison to the other groups, the overall trend was similar although some groups did not follow the exact protocol. The cold germinating peas inhibited oxygen consumption, however they were still respiring since they had to regain energy from their cold environment. According to The Journal of Research in Science and Teaching, it is important to understand that this photosynthetic process relates to cellular respiration because photosynthesis creates the glucose that is

In this experiment, seeds were using oxygen for respiration, so the level of gas in the respirometer decreased. This is why the level of oxygen in the pipettes went down. This happened because of respiration, where cells take in oxygen to make ATP and release carbon dioxide. The carbon dioxide made was absorbed by potassium hydroxide. With the data, the germinating seeds used oxygen, and so did the rest, strangely. The relationship between oxygen consumption and time was an increasing one, so as time passed, more oxygen was used, as shown back in figure 1. Germination causes cellular respiration to occur and though germinating seeds should have been the one who respired most by far, the data collected said otherwise, and this could have been

1), the amount of dissolved oxygen in the solution without NaOH is higher than the amount found in the solution with NaOH. At 90 seconds, the dissolved oxygen in the two solutions only differs from one another about 0.18 (mg/L), a small value compared to the 0.43 (mg/L) of a difference found at 360 seconds. The difference in dissolved oxygen continues to increase as time goes by, leading to 0.87 (mg/L) of a difference at 540 seconds. Analyzing the values individually, the amount of dissolved oxygen in the solution without NaOH displays a consistent increase, while that of the solutions with NaOH displays a fluctuating

The materials and methods sections may be referred to in order to see how these tests were done. Overall, the cold water reaction had less oxygen gas than the warm and boiling water, which are much closer together. All increase steadily. The warm and boiling water leveled off around 130 seconds and increased at a slower rate from there. The rate of increase for warm and boiling water was highest between 10 seconds and 50 seconds. The increase of oxygen in cold water was much more constant, with a minor spike between 60 and 70 seconds.

Cellular respiration is a metabolic process that refers to the creation of intracellular energy in which molecules are oxidized and the final electron acceptor is an inorganic molecule. The process of cellular respiration is further divided into two pathways; anaerobic, which proceeds without the presence of oxygen, and aerobic, which requires oxygen, however, before either form of cellular respiration can occur, the catabolic process of glycolysis must occur as the intermediate products of glycolysis are required to start both aerobic or anaerobic respiration. It should be further noted, that the general term of “cellular respiration” usually refers to aerobic respiration. Glycolysis, sometimes referred to as the Embden-Meyerhof pathway,

The aereobic cellular respiration process happens in four steps and they all have an importance and can not be completed with out all of them involved. The important thing is that it cannot happen without the one that happens before it. Glycolysis is the first step in cellular reperation process. This occurs in the areobic and in the anareobicc ells respiration process. During this step it releases a minute amount of energy.Glycolysis is a model or prototype of what is called the universal mettabolic pathway. It happens in several different ways in all organisms, both aerobic and anaerobic. The vast occurrence of glycolysis tells us that it is one of the oldest known metabolic process.Glycolysis contains ten intermediate compounds, and is