Lab 8 - Chemical and Physical Processes of Digestion

2106 Words Mar 8th, 2011 9 Pages
Lab 8 - Chemical and Physical Processes of Digestion (p.105)

Introduction:

In this lab, I will study how digestion of carbohydrates, proteins, and fats occurs. I will define Key Terms that describe what will occur in the experiments; I will conduct an experiment for each Activity and provide all resulting Data as well as answer Questions from each Activity. I will then provide a short Summary for what I learned in each Activity.

Key Terms:

Enzymes (p. 105) – Speed up chemical reactions in living things. Biological catalysts.

Catalysts (p. 105) - is any substance which makes a chemical reaction go faster, without itself being changed.

Hydrolases (p. 105) – Reaction with water. A chemical process in
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Freeze, Inc. 37 degrees C 37 degrees C 37 degrees C

37 deg C for 60 min 60 min. 60 min. 60 min.

IKI Test - - - +

Benedict's Test ++ ++ ++ -

Chart 2 Enzyme Digestion of Starch and Cellulose (continued)

Tube # 5 6 7

Additives Amylase, Cellulose Peptidase, Starch Bacteria, Cellulose

DI Water pH 7.0 buffer pH 7.0 buffer

Incubation Cond. 37 degrees C 37 degrees C 37 degrees C

60 min. 60 min. 60 min.

IKI Test + + -

Benedict's Test - - ++

Questions:

2-1: Which tubes showed that starch or cellulose was still present? Tubes ___4__, ____5__, __6___

2-2: Which tubes tested positive for the presence of reducing sugars? Tubes__1__, ___2_, __3__, ___7_

2-3: What was the effect of freezing tube 1?

The IKI test shows no starch. The Benedicts Assay shows a high amount of glucose and maltose. This is the same thing for test tube 2, which was same experiment, but not frozen as 1 was. I don’t think there was an effect. The amylase was still able to digest starch.

2-4: How does the effect of freezing

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