Lab 8 - Chemical and Physical Processes of Digestion

2106 WordsMar 8, 20119 Pages
Lab 8 - Chemical and Physical Processes of Digestion (p.105) Introduction: In this lab, I will study how digestion of carbohydrates, proteins, and fats occurs. I will define Key Terms that describe what will occur in the experiments; I will conduct an experiment for each Activity and provide all resulting Data as well as answer Questions from each Activity. I will then provide a short Summary for what I learned in each Activity. Key Terms: Enzymes (p. 105) – Speed up chemical reactions in living things. Biological catalysts. Catalysts (p. 105) - is any substance which makes a chemical reaction go faster, without itself being changed. Hydrolases (p. 105) – Reaction with water. A chemical process in…show more content…
Freeze, Inc. 37 degrees C 37 degrees C 37 degrees C 37 deg C for 60 min 60 min. 60 min. 60 min. IKI Test - - - + Benedict's Test ++ ++ ++ - Chart 2 Enzyme Digestion of Starch and Cellulose (continued) Tube # 5 6 7 Additives Amylase, Cellulose Peptidase, Starch Bacteria, Cellulose DI Water pH 7.0 buffer pH 7.0 buffer Incubation Cond. 37 degrees C 37 degrees C 37 degrees C 60 min. 60 min. 60 min. IKI Test + + - Benedict's Test - - ++ Questions: 2-1: Which tubes showed that starch or cellulose was still present? Tubes ___4__, ____5__, __6___ 2-2: Which tubes tested positive for the presence of reducing sugars? Tubes__1__, ___2_, __3__, ___7_ 2-3: What was the effect of freezing tube 1? The IKI test shows no starch. The Benedicts Assay shows a high amount of glucose and maltose. This is the same thing for test tube 2, which was same experiment, but not frozen as 1 was. I don’t think there was an effect. The amylase was still able to digest starch. 2-4: How does the effect of freezing

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