Lab: Enzymes – Protein Catalysts

858 Words Jun 19th, 2018 4 Pages
Lab: Enzymes – Protein Catalysts

Abstract: Enzymes are catalysts therefore we can state that they work to start a reaction or speed it up. The chemical transformed due to the enzyme (catalase) is known as the substrate. In this lab the chemical used was hydrogen peroxide because it can be broken down by catalase. The substrate in this lab would be hydrogen peroxide and the enzymes used will be catalase which is found in both potatoes and liver. This substrate will fill the active sites on the enzyme and the reaction will vary based on the concentration of both and the different factors in the experiment. Students placed either liver or potatoes in test tubes with the substrate and observed them at different temperatures as well as with
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3. a) The rate of enzyme activity is the highest at 37 degrees. The rate of enzyme activity is the lowest at 100 degrees.

b) Enzyme reaction is very slow due to the fact that the molecules have low to no kinetic energy. Molecular collision is minimal because molecules do not have enough activation energy for the reaction to occur. The increase in temperature causes particles to move around faster (more kinetic energy) therefore causing them to collide more often thus speed up the reaction. The lowered temperature causes molecules to collide less frequently, therefore delaying the reaction. The rate of enzyme activity for this particular enzyme is 37 degrees (Birju Amin , 2003) which means that after this particular temperature the enzyme will begin to denature, therefore the reaction activity decreases after this temperature.

c) Every substrate has an optimum temperature for its enzyme. This temperature can vary depending on other factors such as how long the enzyme was exposed to this temperature. If the enzyme is only exposed to the extreme temperature for a short period of time, it has the capability of surpassing its optimum temperature. In the experiment performed, the liver was placed in a beaker of boiling water for approximately 10 minutes thus not allowing its optimum to exceed its norm. (Jim Clark, 2007).

4. a) The rate of enzyme activity is the highest at a pH of 8. The enzyme reactivity is at 4.6. At the two extremes on the pH scale,

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